Gene Therapy Protocols

Significant advances in gene transfer technology and the completion of sequencing the human genome offer much renewed hope that gene therapy will provide novel approaches to the treatment of inherited and acquired diseases. In this fully updated and revised 2nd edition of the warmly received Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. The methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. In addition, there are completely revised methods based on retroviruses and adenoviruses, as well as new and promising methods based on lentiviruses and adeno-associated viruses. The quantitative aspects of gene therapy are strongly emphasized, and the book reflects the many significant recent improvements in gene transfer efficiency, gene regulation, and vector production.
Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven step-by-step laboratory procedures that enable successful therapeutic application.

From Reviews of the First Edition:
"...extremely impressive...a must, even for researchers who are already familiar with gene transfer techniques..." -Molecular Medicine Today

"...succeeds admirably..."-Genetical Research

From Reviews of the Second Edition:

"Each protocol is written in a standard format, consisting of introduction, materials, methods, notes, and references. The introductions vary in length and detail. Many of them are illustrated with tables, charts, schematics, and/or photographs. The materials and methods sections are quite detailed, and there are many notes clarifying steps or outlining potential problems. Gene Therapy Protocols is recommended for university, hospital, and corporate libraries serving researchers who are working on gene therapies." - E-STREAMS

"All the chapters are well written, and the protocols are easy to understand. This book is applicable to those directly involved in the field of gene therapy." - Pharmaceutical Research

"...a valuable resource for background information in the field of gene transfer vector development, as well as providing concise and clearly written protocols for the production of these vectors." - Molecular Biotechnology

"Each of the 32 chapters is constructed like a cooking-book with detailed instructions and illustrations of how to perform the procedures. For anyone who is planning or is already practically involved in gene therapy experiments, this impressive book is a must in the laboratory." - Acta Derm Venereol

"Second edition of the "Gene Therapy Protocols" in successful series of books "Methods in Molecular Medicine" from Humana Press, is certainly a publication, which appeared in the right time. The comprehensibility of the book represents great value also for researcher. This collection of gene therapy protocols is a valuable and indispensable resource for basic scientists, graduate students, and postdoctoral fellows. Even clinical researchers will find the book very informative and useful. The book represents an outstanding work, which should not be missed in all laboratories dealing with gene therapy." - Neoplasma