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  • © 2007

Plant Secondary Metabolites

  • Reliable assays meant to equip researchers to meet the challenge of fulfilling the huge demand for feed
  • Provides information for readers to gain a better understanding of plant-animal interactions
  • Methods for analysis of some important plant secondary metabolites
  • Methods written in a recipe-like format designed for direct practical use in the laboratory
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 393)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Trypsin Inhibitor

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 1-6
  3. Chymotrypsin Inhibitor

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 7-9
  4. α-Amylase Inhibitor

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 11-14
  5. Phytohemagglutin/Lectin

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 15-21
  6. Phytic Acid

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 23-27
  7. Oxalic Acid

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 29-32
  8. Nitrate and Nitrite

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 33-39
  9. L-Mimosine (β-(3-Hydroxy-4-Pyridone-1-yl)-L-Alanine)

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 41-46
  10. Canavanine

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 47-49
  11. L-DOPA (L-3,4-Dihydroxyphenylalanine)

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 51-53
  12. Glucosinolates

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 55-60
  13. Cyanogenic Glucosides/Cyanogens

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 61-65
  14. Tannins

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 67-81
  15. Gossypol

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 83-88
  16. Chlorogenic Acid

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 89-91
  17. Saponins

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 93-100
  18. Phorbol Esters

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 101-105
  19. Alkaloids

    • Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker
    Pages 107-111
  20. Back Matter

    Pages 113-130

About this book

Plant Secondary Metabolites provides reliable assays that will equip researchers to meet the unprecedented challenge of fulfilling the huge demand for feed driven by increasing demand of animal protein in developing countries. Using more than a decade of experience working with the quantification of plant secondary metabolites, the authors have created a manual allowing for the safe and efficient use of locally available feed resources. This comprehensive new volume also offers readers a better understanding of plant-animal interactions. The methodologies provided in this volume may also be used to determine plant secondary metabolites in human food and in studying the implications of their consumption on human health and welfare.

This volume contains methods for analysis of some important plant secondary metabolites, which may be conducted in laboratories equipped with basic facilities. The chemical nature of plant secondary metabolites and their known physiological effects and mechanisms of action are also briefly presented. The methods have been written in a recipe-like format designed for direct practical use in the laboratory. Authoritative and extensive, Plant Secondary Metabolites will become an essential reference for plant biologists, biochemists, and molecular and cell biologists.

Reviews

From the reviews:

"The entire book is written by three authors … . the authors have produced a succinct series of methodologies in this short 18-chapter, 130-page volume. … The chapters cover 18 target compounds or compound groups. … There is a clear audience for these protocols, and I am sure they will be happy with the result." (Dr Robert Hall, Molecular Biotechnology, Vol. 38, 2008)

Authors and Affiliations

  • Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim, Stuttgart, Germany

    Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker

Bibliographic Information

  • Book Title: Plant Secondary Metabolites

  • Authors: Harinder P. S. Makkar, P. Siddhuraju, Klaus Becker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-425-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-993-2Published: 06 July 2007

  • Softcover ISBN: 978-1-61737-872-0Published: 19 November 2010

  • eBook ISBN: 978-1-59745-425-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 130

  • Number of Illustrations: 18 b/w illustrations

  • Topics: Plant Sciences, Cell Biology, Plant Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access