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Methods in Molecular Biology

Genetic Modification of Hematopoietic Stem Cells

Methods and Protocols

Editors: Baum, Christopher (Ed.)

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  • Provides an easily accessible reference volume for genetic modification of hematopoietic cells using various state-of-the-art vector systems
  • Comprehensive guide on functional, phenotypic and genotypic screening of gene-modified cells written by leading researchers in the field
  • Contains helpful insights into preclinical data acquisition and documentation for gene therapy, including regulatory comments from the EU and US perspectives
  • Covers a wide range of cell-based and animal assay formats and systems
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59745-409-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-58829-980-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61737-866-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls.

Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells.

Reviews

From the reviews:

"This book provides a unique and comprehensive resource of protocols for the genetic modification of various hematopoietic cell types and up-to-date procedures for molecular and systemic monitoring. … This book is a great help for the design of excellent research in basic hematology, oncology, genetics, and immunology, and also promote the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells. The book represents an outstanding work, which should not be missed in all biomedical research laboratories dealing with gene therapy of hematopoietic cells." (C. Altaner, Neoplasma, June, 2009)

"This book provides a unique and comprehensive resource of protocols for the genetic modification of various hematopoietic cell types and up-to-date procedures for molecular and systemic monitoring. … This book is a great help for the design of excellent research in basic hematology, oncology, genetics, and immunology, and also promote the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells. The book represents an outstanding work, which should not be missed in all biomedical research laboratories dealing with gene therapy of hematopoietic cells." (C. Altaner, Neoplasma, April, 2009)

“Haematopoietic stem cells are attractive targets for gene therapy. … Each chapter provides a brief review of the area, followed by a step-by-step guide to undertaking experiments and includes details of reagents required and a list of useful notes or comments of issues that may arise. Comprehensive reference citations provide sources of further information when required. The book should provide a road map for researchers, clinical investigators and regulators involved in modifying haematopoietic cells.”­­­ (Waseem Qasim, Human Genetics, Vol. 128, May, 2010)


Table of contents (32 chapters)

Table of contents (32 chapters)
  • Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications

    Pages 1-11

    Guenechea, Guillermo (et al.)

  • Isolation of Human and Mouse Hematopoietic Stem Cells

    Pages 13-21

    Ng, Yuk Yin (et al.)

  • Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors

    Pages 23-31

    Modlich, Ute (et al.)

  • Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials

    Pages 33-58

    Budak-Alpdogan, Tulin (et al.)

  • Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer

    Pages 59-70

    Sio, Francesca Santoni de (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59745-409-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-58829-980-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61737-866-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Genetic Modification of Hematopoietic Stem Cells
Book Subtitle
Methods and Protocols
Editors
  • Christopher Baum
Series Title
Methods in Molecular Biology
Series Volume
506
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-409-4
DOI
10.1007/978-1-59745-409-4
Hardcover ISBN
978-1-58829-980-2
Softcover ISBN
978-1-61737-866-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XXII, 490
Number of Illustrations
74 b/w illustrations, 10 illustrations in colour
Topics