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Methods in Molecular Biology

Macrophages and Dendritic Cells

Methods and Protocols

Editors: Reiner, Neil E. (Ed.)

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  • Provides an easily accessible reference volume for state-of-the-art experimentation by leading researchers in the field
  • Covers a wide range of approaches for the isolation and characterization of macrophages and dendritic cells
  • Provides detailed step-by-step protocols to modify gene expression in cells that are notoriously resistant to genetic manipulation
  • Provides approaches for detailed quantitative functional analyses including activation states and lipid signaling
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eBook $129.00
price for USA in USD
  • ISBN 978-1-59745-396-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
Softcover $169.00
price for USA in USD
About this book

In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.

Reviews

From the reviews: “This book, part of the Methods in Molecular Biology series, provides detailed descriptions of techniques that … may be adapted to individual research projects. … a book on state-of-the-art approaches to investigate the roles of macrophages and dendritic cells in health and disease to better understand their function in the host response. … useful for graduate students, postdoctoral fellows, and experienced investigators. … the protocols are described in great detail in this book, which should be helpful to anyone considering establishing a new research procedure.” (Marion C. Cohen, Doody’s Review Service, December, 2009)

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors

    Pages 1-15

    Schäfer, Georgia (et al.)

  • Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions

    Pages 17-27

    Mortellaro, Alessandra (et al.)

  • Macrophage Activation: Classical Vs. Alternative

    Pages 29-43

    Classen, Andrea (et al.)

  • Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy

    Pages 45-56

    Steinberg, Benjamin E. (et al.)

  • Detection of Activated Rab7 GTPase with an Immobilized RILP Probe

    Pages 57-69

    Sun, Jim (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-59745-396-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
Softcover $169.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Macrophages and Dendritic Cells
Book Subtitle
Methods and Protocols
Editors
  • Neil E. Reiner
Series Title
Methods in Molecular Biology
Series Volume
531
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-396-7
DOI
10.1007/978-1-59745-396-7
Hardcover ISBN
978-1-58829-972-7
Softcover ISBN
978-1-62703-849-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 368
Topics