Methods in Molecular Biology

Cyclooxygenases

Methods and Protocols

Editors: Ayoub, Samir S., Flower, Roderick J., Seed, Michael (Eds.)

  • Serves as a detailed step-by-step laboratory manual covering the most up-to-date, widely used protocols to study the biology of the cyclooxygenase enzymes
  • Includes chapters written by some of the pioneers in the cyclooxygenase field
  • Acts as a reference that no cyclooxygenase or prostanoid research laboratory should be without
  • An indispensable tool for pharmaceutical companies engaged in the discovery and development of cyclooxygenase inhibitors
  • Describes molecular and biochemical techniques used to study the purification, cloning, expression, and activity of the cyclooxygenase enzymes
see more benefits

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-364-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-953-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5638-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Since the discovery of the pharmacological and toxicological importance of inhibiting the cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs (NSAIDs), much research has gone into the development of methods to study the biological functions of COX-1 and COX-2.  In Cyclooxygenases: Methods and Protocols, experts and pioneers in the field present the most up-to-date in vitro and in vivo techniques routinely used in COX research.  The volume delves into essential topics such as the purification, cloning, and expression of COX enzymes as well as in vitro assays aimed at determining the inhibitory potency of drugs on COX-1 and COX-2 activities, with chapters describing protocols used for the extraction and measurement of the prostanoids. This volume also describes in vivo disease models used to study the roles of COX-1 and COX-2 in gastrointestinal injury, inflammation, and pain.  As a book in the highly successful Methods in Molecular Biology™ series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cyclooxygenases: Methods and Protocols serves as an indispensable tool for all scientists seeking the treatment of inflammation, pain, fever, and other harmful conditions.

Table of contents (18 chapters)

  • In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I

    Flower, Roderick J.

    Pages 3-10

  • Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites

    Inoue, Hiroyasu (et al.)

    Pages 11-20

  • Purification of Recombinant Human COX-1 and COX-2

    Gierse, James K.

    Pages 21-29

  • Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2

    Staten, Nicholas R. (et al.)

    Pages 31-43

  • Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System

    Hunter, John C. (et al.)

    Pages 45-54

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-364-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-953-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5638-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Cyclooxygenases
Book Subtitle
Methods and Protocols
Editors
  • Samir S. Ayoub
  • Roderick J. Flower
  • Michael Seed
Series Title
Methods in Molecular Biology
Series Volume
644
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-364-6
DOI
10.1007/978-1-59745-364-6
Hardcover ISBN
978-1-58829-953-6
Softcover ISBN
978-1-4939-5638-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 218
Topics