- Provides easy to follow protocols in this technically challenging field
- Presents a cohesive review of a variety of methods to isolate, identify, and study cancer stem cells
- A timely contribution in this nascent field, the book will level the playing field of investigators of cancer stem cells
- Presents a comprehensive, step-by-step guide by leading researchers in the field of cancer stem cells
- Brings to light methods that have been mired in mystery
- Brings together divergent viewpoints and methods to isolate the same population of cells, from prospective isolation using surface markers to stem cell propagation assays
Buy this book
- About this book
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Through the revolutionary concept of cancer stem cells, cancer research has been reinvigorated to study the role of these unique cells in cancer propagation and as targets of innovative therapies. In Cancer Stem Cells: Methods and Protocols, preeminent researchers have compiled cancer stem cell research techniques and protocols to promote healthy competition, discourse, and collaboration in this vital field. The volume covers extensive topics such as identification and isolation of cancer stem cells, animal models of cancer stem cells, methylation profiling, the contribution of the niche in the regulation of cancer stem cells, immunologic targeting, and the use of normal stem cells as a treatment, among other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Cancer Stem Cells: Methods and Protocols will aid scientists around the world in the furthering of our understanding of cancer initiation and propagation and, most importantly, in the development of novel targets for cancer therapy.
- Reviews
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From the reviews:
“This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. … The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. … The topic is fascinating and clinically relevant.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)
- Table of contents (17 chapters)
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Defining Cancer Stem Cells by Xenotransplantation in Zebrafish
Pages 1-5
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Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models
Pages 7-19
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Identification of Murine and Human Acute Myeloid Leukemia Stem Cells
Pages 21-35
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Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal
Pages 37-56
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Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133
Pages 57-71
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Table of contents (17 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Cancer Stem Cells
- Book Subtitle
- Methods and Protocols
- Editors
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- John S. Yu
- Series Title
- Methods in Molecular Biology
- Series Volume
- 568
- Copyright
- 2009
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59745-280-9
- DOI
- 10.1007/978-1-59745-280-9
- Hardcover ISBN
- 978-1-58829-938-3
- Softcover ISBN
- 978-1-61779-753-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XVI, 284
- Number of Illustrations
- 42 b/w illustrations, 16 illustrations in colour
- Topics
