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Methods in Molecular Biology

Two-Dimensional Electrophoresis Protocols

Editors: Sheehan, David, Tyther, Raymond (Eds.)

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  • Covers a wide biological range of proteome sources including microbes, plants and animals
  • Addresses key aspects of experimental design
  • Includes some of the latest approaches such as DIGE and detection of post-translational modification
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  • ISBN 978-1-59745-281-6
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About this book

Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.

Table of contents (36 chapters)

Table of contents (36 chapters)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-59745-281-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA in USD
  • ISBN 978-1-58829-937-6
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Online orders shipping within 2-3 days.
Softcover $199.00
price for USA in USD
  • ISBN 978-1-61737-849-2
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Two-Dimensional Electrophoresis Protocols
Editors
  • David Sheehan
  • Raymond Tyther
Series Title
Methods in Molecular Biology
Series Volume
519
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-281-6
DOI
10.1007/978-1-59745-281-6
Hardcover ISBN
978-1-58829-937-6
Softcover ISBN
978-1-61737-849-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 546
Number of Illustrations
99 b/w illustrations, 1 illustrations in colour
Topics

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