Methods in Molecular Biology

Prion Protein Protocols

Editors: Hill, Andrew (Ed.)

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  • Most current collection of protocols in prion disease research
  • Fully described methods chapters from leaders in the field with easy to follow instructions and notes sections
  • Provides methods for studying prion infection in cell cultures
  • Detailed protocols for prion protein conversion assays using current technologies
  • Comprehensive chapter on the molecular diagnosis of human prion diseases
  • Reviews of the use of transgenic mouse models of prion diseases
  • Methods for the biochemical characterization of mammalian prion proteins
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  • ISBN 978-1-59745-234-2
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Hardcover $159.99
price for USA in USD
  • ISBN 978-1-58829-897-3
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Softcover $159.00
price for USA in USD
  • ISBN 978-1-61737-831-7
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  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
About this book

While much information has been gained over the last two decades regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form. In Prion Protein Protocols, an international team of experts provide an up-to-date collection of current methods in this unique area of neuroscience.  The chapters contained in this volume, both protocols and useful review chapters, feature topics such as the cell biology of prions, techniques and approaches to studying prion infection in cultured cells, how these systems can be used as a rapid bioassay, and prion protein misfolding. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include readily reproducible, step-by-step laboratory protocols, lists of the necessary materials and reagents, and the Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Prion Protein Protocols is an ideal collection for researchers investigating the growing field of mammalian prion disease.

Reviews

From the reviews:

"Interspersed are concise reviews focusing on current growth areas in the prion field. … The protocols are clear and authoritative. For those entering the field it would be an excellent guide." (Hugh Pennington, Microbiology Today, February, 2009)


Table of contents (18 chapters)

Table of contents (18 chapters)
  • Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases

    Pages 1-20

    Bedecs, Katarina

  • Investigation of PrPC Metabolism and Function in Live Cells

    Pages 21-34

    Haigh, Cathryn L. (et al.)

  • Immunodetection of PrPSc Using Western and Slot Blotting Techniques

    Pages 35-48

    Gyllberg, Hanna (et al.)

  • Assaying Prions in Cell Culture

    Pages 49-68

    Mahal, Sukhvir P. (et al.)

  • Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes

    Pages 69-82

    Vella, Laura J. (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-59745-234-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-58829-897-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $159.00
price for USA in USD
  • ISBN 978-1-61737-831-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Prion Protein Protocols
Editors
  • Andrew Hill
Series Title
Methods in Molecular Biology
Series Volume
459
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-234-2
DOI
10.1007/978-1-59745-234-2
Hardcover ISBN
978-1-58829-897-3
Softcover ISBN
978-1-61737-831-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 280
Number of Illustrations
38 b/w illustrations, 5 illustrations in colour
Topics