Get 40% off our selection of bestselling print books in Engineering through October 31st!

Methods in Molecular Biology

Mycobacteria Protocols

Editors: Parish, Tanya, Brown, Amanda Claire (Eds.)

  • Fully updated from the first edition to offer cutting edge methods for manipulation of Mycobacteria
  • Includes methods for preparation and analysis of sub-cellular fractions
  • Provides description of genetic systems and instructions for manipulating the genome
  • Contains techniques for studying gene expression, singly and globally, and updated techniques for mutagenesis and gene essentiality testing
  • With detailed protocols for genomic, transcriptomic and proteomic analysis, and new molecular methods for typing and drug susceptibility profiling
see more benefits

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-207-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-889-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61737-826-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.  Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field.  Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.

Table of contents (28 chapters)

  • Isolation of Mycobacterium Species Genomic DNA

    Belisle, John T. (et al.)

    Pages 1-12

  • Isolation of Mycobacterial RNA

    Rustad, Tige R., PhD (et al.)

    Pages 13-22

  • Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization

    Shi, Libin, PhD (et al.)

    Pages 23-45

  • Analyzing Lipid Metabolism: Activation and β-Oxidation of Fatty Acids

    Wheeler, Paul Robert, Ph.D

    Pages 47-59

  • Analysis of Lipid Biosynthesis and Location

    Wheeler, Paul Robert, Ph.D

    Pages 61-81

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-207-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-889-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61737-826-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Mycobacteria Protocols
Editors
  • Tanya Parish
  • Amanda Claire Brown
Series Title
Methods in Molecular Biology
Series Volume
465
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-207-6
DOI
10.1007/978-1-59745-207-6
Hardcover ISBN
978-1-58829-889-8
Softcover ISBN
978-1-61737-826-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIX, 456
Additional Information
Originally published as volume 101 in this series
Topics