Editors:
- Synthesizes electroporation, electroporation chemotherapy, and electroporation gene therapy, with a focus on the latter
- Contains step-by-step instructions that ensure successful results
- Provides notes on troubleshooting and avoiding known pitfalls
Part of the book series: Methods in Molecular Biology (MIMB, volume 423)
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Table of contents (40 protocols)
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Front Matter
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Basic Concepts of DNA Transfer via Electroporation
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In Vitro Targeted Gene Delivery via Electroporation
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In Vivo Targeted Gene Delivery via Electroporation
About this book
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
Editors and Affiliations
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Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, USA
Shulin Li
Bibliographic Information
Book Title: Electroporation Protocols
Book Subtitle: Preclinical and Clinical Gene Medicine
Editors: Shulin Li
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-194-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-877-5Published: 01 February 2008
Softcover ISBN: 978-1-61737-820-1Published: 10 December 2010
eBook ISBN: 978-1-59745-194-9Published: 28 March 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXII, 524
Number of Illustrations: 141 b/w illustrations, 1 illustrations in colour
Topics: Biochemistry, general, Animal Genetics and Genomics, Cell Biology, Animal Biochemistry, Biotechnology