Methods in Molecular Biology

Chromatin Protocols

Editors: Chellappan, Srikumar P. (Ed.)

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  • Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques
  • Includes detailed, step-by-step guidelines for the analysis of chromatin structure and its role in transcription, replication and other biological processes
  • Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination
  • Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins
  • Acts as a comprehensive guide to the most up-to-date techniques dealing with all aspects of chromatin biology
  • Presents experimental details in an easy to follow modular format typical of the MiMB series
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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-190-1
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-873-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5626-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA.  In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function.  Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.

Reviews

From the reviews of the second edition: “This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. … In summary, this is a very useful text, with lots of strengths … .” (Ray Waters, Human Genetics, Vol. 126, 2009)

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos

    Bonte, Edgar (et al.)

    Pages 1-10

  • Analysis of Reconstituted Chromatin Using a Solid-Phase Approach

    Sandaltzopoulos, Raphael (et al.)

    Pages 11-25

  • In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins

    Winter, Sherry L. (et al.)

    Pages 27-40

  • DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules

    Pardo, Carolina (et al.)

    Pages 41-65

  • Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues

    Furusawa, Takashi (et al.)

    Pages 67-82

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-190-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-873-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5626-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chromatin Protocols
Editors
  • Srikumar P. Chellappan
Series Title
Methods in Molecular Biology
Series Volume
523
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-190-1
DOI
10.1007/978-1-59745-190-1
Hardcover ISBN
978-1-58829-873-7
Softcover ISBN
978-1-4939-5626-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 416
Topics