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Methods in Molecular Biology

SARS- and Other Coronaviruses

Laboratory Protocols

Editors: Cavanagh, Dave (Ed.)

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  • Presents 23 state-of-the-art, widely applicable protocols with minute detail
  • Written by international experts from 20 institutions
  • Relevant to a wide array of professions
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  • ISBN 978-1-59745-181-9
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About this book

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.

Reviews

From the reviews:

"The serious nature of coronaviruses became well known with the discovery of the severe acute respiratory syndrome (SARS) virus which spread rapidly with life-threatening consequences in humans. ... this book describes the laboratory techniques used to study them. ... Scientists and students working in virology laboratories and, specifically, laboratories working with coronaviruses are the intended audience. ... each chapter contains a copious amount of detailed information as well as specific notes with technical advice. This is a valuable resource for scientists working with coronaviruses." (Rebecca T. Horvat, Doody's Review Service, November, 2008)

"This is volume 454 in the series Methods in Molecular Biology. … if you work on coronaviruses, or want to enter the field, this is a must-have book at an affordable price." (Stuart Siddell, Microbiology Today, February, 2009)


Table of contents (23 chapters)

Table of contents (23 chapters)
  • A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses

    Pages 3-12

    Vijgen, Leen, PhD (et al.)

  • Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions

    Pages 13-26

    Poon, Leo L.M., DPHIL (OXON) (et al.)

  • Detection and Sequence Characterization of the 3′-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m

    Pages 27-34

    Jonassen, Christine Monceyron, PhD

  • RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot

    Pages 35-42

    Culver, Francesca Anne, BSC, PhD (et al.)

  • Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains

    Pages 43-59

    Hasoksuz, Mustafa, PhD (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-181-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-58829-867-6
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61737-815-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
SARS- and Other Coronaviruses
Book Subtitle
Laboratory Protocols
Editors
  • Dave Cavanagh
Series Title
Methods in Molecular Biology
Series Volume
454
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-181-9
DOI
10.1007/978-1-59745-181-9
Hardcover ISBN
978-1-58829-867-6
Softcover ISBN
978-1-61737-815-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 326
Number of Illustrations
64 b/w illustrations
Topics

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