Methods in Molecular Biology

Glycomics

Methods and Protocols

Editors: Packer, Nicolle H., Karlsson, Niclas G. (Eds.)

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  • Presents a detailed compendium of techniques used routinely in leading laboratories utilizing current, common technologies, especially those established for proteomics research
  • Reviews the current practices used for the analysis of the specific single O-GlcNAc found on nuclear and cytoplasmic proteins
  • Disperses the misconception that only experts can analyze the glycome
  • Inspires researchers to pursue glycan function through sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools
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  • ISBN 978-1-59745-022-5
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Hardcover $179.00
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About this book

Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.

Reviews

From the reviews: “This book discusses methods of a comparatively new direction in science, which has been named glycomics. … The book consists two parts with 26 chapters prepared by well-known experts from many countries. … I confirm the book fulfills the goal and I consider this book as an excellent practical guide to glycomics methods. Each method is described in great detail, and the whole book is excellent manual for many researchers in biochemistry, molecular biology, immunochemistry, biotechnology, and medicine.” (G. Wiederschain, Biochemistry, Vol. 75 (4), 2010)

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Analysis of N- and O-Linked Glycans from Glycoproteins Using MALDI-TOF Mass Spectrometry

    Pages 3-21

    Morelle, Willy (et al.)

  • Infrared Multiphoton Dissociation Mass Spectrometry for Structural Elucidation of Oligosaccharides

    Pages 23-35

    Li, Bensheng (et al.)

  • Mass Spectrometry of N-Linked Glycans

    Pages 37-51

    Azadi, Parastoo (et al.)

  • Solid-Phase Permethylation for Glycomic Analysis

    Pages 53-64

    Mechref, Yehia (et al.)

  • Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization

    Pages 65-77

    Lattová, Erika (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-022-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Glycomics
Book Subtitle
Methods and Protocols
Editors
  • Nicolle H. Packer
  • Niclas G. Karlsson
Series Title
Methods in Molecular Biology
Series Volume
534
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-022-5
DOI
10.1007/978-1-59745-022-5
Hardcover ISBN
978-1-58829-774-7
Softcover ISBN
978-1-61737-769-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 390
Topics