Methods in Molecular Biology

Mouse Cell Culture

Methods and Protocols

Editors: Ward, Andrew, Tosh, David (Eds.)

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  • Features methods involving a wide variety of tissue types in one convenient volume
  • Written by leading experts in a step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences
  • Covers protocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively
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eBook $129.00
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  • ISBN 978-1-59745-019-5
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Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-772-3
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  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
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  • ISBN 978-1-4939-6086-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Reviews

From the reviews:

“This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. … This book is recommended for researchers and students who are interested in studying the culture of cells from animal models.” (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)

Table of contents (18 chapters)

  • The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies

    Jackson, Melany (et al.)

    Pages 1-18

  • Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures

    Garfield, Alastair S.

    Pages 19-27

  • Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

    Smith, Janet (et al.)

    Pages 29-56

  • The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference

    Davies, Jamie A.

    Pages 57-69

  • Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation

    del Moral, Pierre-Marie (et al.)

    Pages 71-79

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59745-019-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-58829-772-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6086-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mouse Cell Culture
Book Subtitle
Methods and Protocols
Editors
  • Andrew Ward
  • David Tosh
Series Title
Methods in Molecular Biology
Series Volume
633
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-59745-019-5
DOI
10.1007/978-1-59745-019-5
Hardcover ISBN
978-1-58829-772-3
Softcover ISBN
978-1-4939-6086-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 257
Topics