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Methods in Molecular Biology

Free Radicals and Antioxidant Protocols

Editors: Uppu, R.M., Murthy, S.N., Pryor, W.A., Parinandi, N.L. (Eds.)

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  • Comprised of twenty-seven chapters contributed by world-renowned experts in the field
  • Fully updated from the first edition to offer cutting-edge methods for the rapid detection of RONs and redox stress
  • Includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60327-029-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-58829-710-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61779-683-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.

Reviews

From the reviews of the second edition:

“The book is organized into four sections which draw on contributions from several experts in the field of oxidative stress. … Based on a collection of articles in the four sections, the book is a good reference for those interested in free radical science including new methodologies to measure both oxidative stress and antioxidants using in vivo and in vitro models.” (Silvia Berlanga de Moraes Barros, Brazilian Journal of Pharmaceutical Sciences, May, 2010)

Table of contents (27 chapters)

Table of contents (27 chapters)
  • In Vivo Imaging of Free Radicals and Oxygen

    Pages 3-27

    Vikram, Deepti S. (et al.)

  • In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle, Lithium Phthalocyanine

    Pages 29-39

    Hyodo, F. (et al.)

  • Measurement of Plasma Nitrite by Chemiluminescence

    Pages 41-49

    Nagababu, Enika (et al.)

  • Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species

    Pages 51-61

    Sathishkumar, K. (et al.)

  • Phenolic Acids and Flavonoids: Occurrence and Analytical Methods

    Pages 65-90

    Stalikas, Constantine D.

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60327-029-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-58829-710-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61779-683-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Free Radicals and Antioxidant Protocols
Editors
  • Rao M. Uppu
  • Subramanyam N. Murthy
  • William A. Pryor
  • Narasimham L. Parinandi
Series Title
Methods in Molecular Biology
Series Volume
610
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: Mehul Book Sales, Mumbai, India
eBook ISBN
978-1-60327-029-8
DOI
10.1007/978-1-60327-029-8
Hardcover ISBN
978-1-58829-710-5
Softcover ISBN
978-1-61779-683-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 480
Number of Illustrations
104 b/w illustrations, 11 illustrations in colour
Additional Information
Originally published as volume 108 in the series: Methods Molecular Biology
Topics