Methods in Molecular Biology

Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

Editors: Marx, Andreas, Seitz, Oliver (Eds.)

Free Preview
  • Shows a diverse set of instructive examples from probe design to applications in clinical settings guided by experts in the field who provide easy to follow experiments
  • The book commences with an introduction to the basic principles of fluorescence
  • Extends the potential of realtime PCR
  • Contributions advocate simplifications of the probe technologies
  • Presents a new simplified molecular beacon design EasyBeacons and demonstrates the utility in DNA methylation profiling
see more benefits

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-60327-040-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-58829-700-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-61737-726-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Basic Principles of Fluorescence and Energy Transfer

    Pages 3-19

    Morrison, Larry E., PhD

  • Multiplex Detection of Mutations

    Pages 23-31

    Perlin, David S., PhD (et al.)

  • Detection of Salmonella Species in Foodstuffs

    Pages 33-43

    Bhagwat, Arvind A., PhD (et al.)

  • Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR

    Pages 45-58

    Schmidtke, Gunter (et al.)

  • Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma

    Pages 59-72

    Lukasiak, Sebastian (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-60327-040-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-58829-700-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-61737-726-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols
Editors
  • Andreas Marx
  • Oliver Seitz
Series Title
Methods in Molecular Biology
Series Volume
429
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-040-3
DOI
10.1007/978-1-60327-040-3
Hardcover ISBN
978-1-58829-700-6
Softcover ISBN
978-1-61737-726-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 266
Number of Illustrations
60 b/w illustrations, 17 illustrations in colour
Topics