Neuroprotection Methods and Protocols

1. Front Matter

   Pages i-xii

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2. Neuronal Death and Neuroprotection: A Review

   • Mariaelena Repici, Jean Mariani, Tiziana Borsello

   Pages 1-14

3. The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures

   • Anthony C. Lau, Hong Cui, Michael Tymianski

   Pages 15-29

4. A Modified Silver Technique (de Olmos Stain) for Assessment of Neuronal and Axonal Degeneration

   • Tatyana I. Tenkova, Mark P. Goldberg

   Pages 31-39

5. Model of Acute Injury to Study Neuroprotection

   • Michal Schwartz, Jonathan Kipnis

   Pages 41-53

6. Organotypic Entorhino-Hippocampal Slice Cultures—A Tool to Study the Molecular and Cellular Regulation of Axonal Regeneration and Collateral Sprouting In Vitro

   • Domenico Del Turco, Thomas Deller

   Pages 55-66

7. Role of Nrf2-Dependent ARE-Driven Antioxidant Pathway in Neuroprotection

   • Jiang Li, Marcus J. Calkins, Delinda A. Johnson, Jeffrey A. Johnson

   Pages 67-78

8. Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease States

   • Jennifer Zechel, W. David Lust, Michele Puchowicz

   Pages 79-98

9. A Method for Isolating Prosurvival Targets of NF-κB/Rel Transcription Factors

   • Christian Kuntzen, Francesca Zazzeroni, Can G. Pham, Salvatore Papa, Concetta Bubici, James R. Knabb et al.

   Pages 99-124

10. Functional Cloning of Genes Regulating Apoptosis in Neuronal Cells

    • Roberta Visconti, Luciano D’Adamio

    Pages 125-131

11. Characterization of mRNA Expression in Single Neurons

    • David M. Lin, Brandon Loveall, John Ewer, David L. Deitcher, Nikolaus J. Sucher

    Pages 133-152

12. Brain on a Chip: A Method to Detect Novel Neuroprotective Candidate Targets

    • Yang Tang, Myriam Bernaudin

    Pages 153-166

13. Intracerebral Infusion of Neurotrophic Factors

    • Theo Hagg

    Pages 167-180

14. Synthesis of Cell-Penetrating Peptides and Their Application in Neurobiology

    • Gunnar P. H. Dietz, Mathias Bähr

    Pages 181-198

15. The Stem Cells as a Potential Treatment for Neurodegeneration

    • Ferrari Daniela, Angelo Luigi Vescovi, Daniele Bottai

    Pages 199-213

16. In Vivo Tomographic Imaging Studies of Neurodegeneration and Neuroprotection: A Review

    • Rikki N. Waterhouse, Jun Zhao

    Pages 215-233

17. Back Matter

    Pages 235-239

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Neuroprotection is a topic of great importance in current neuroscience, both basic and clinical. The incidence of age-related neurodegenerative diseases could be expected to rise dramatically in the future owing to an aging population. Consequently, finding the means of retarding or preventing the progression of such diseases becomes increasingly important. This book focuses on basic perspective on neuroprotective approaches and scientists well recognized for their work have contributed chapters to this volume. Although findings on neuroprotection in the different pathologies become more and more frequent and detailed, it can be difficult for researchers to orient themselves in such a complicate field. For this reason, this book describes basic science discovery and the application of such research within different laboratories leading to the development of neuroprotective protocols. The main aim of this volume is thus to give an overview of methods used to study neuronal death and neuroprotection and to offer a really comprehensive step-by-step method in order to make clear not just the procedure but also the principles behind the use of it. At this purpose, the “Notes” section of each chapter represents a useful tool to solve technical problems and to help in reproducing the described methods.

• Alzheimer
• Endoplasmatisches Reticulum
• Parkinson
• Vivo
• genes
• molecular mechanisms
• neurobiology
• neurons
• regulation
• transcription

From the reviews:

"Research in neuroprotection is essential for the development of therapies for stroke and neurodegenerative diseases. This book reviews research methods currently used for prevention and slowing of central nervous system degenerative disorders. … Neurologists, neurosurgeons, and neuroscientists interested in research in neuroprotection are the main audience for this book. This is a comprehensive approach to research in neuroprotection. … the book is well written and structured with no drawbacks. ... a good book for neuroscience and neurological libraries." (Celso Agner, Doody’s Review Service, December, 2007)

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