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Methods in Molecular Biology

Molecular Motors

Methods and Protocols

Editors: Sperry, Ann O. (Ed.)

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  • Provides step-by-step instruction
  • Includes a detailed Notes section
  • Based on the hands-on experience of the authors
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eBook $119.00
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  • ISBN 978-1-59745-490-2
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Hardcover $159.99
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  • ISBN 978-1-58829-665-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
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  • ISBN 978-1-61737-706-8
  • Free shipping for individuals worldwide
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About this book

Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.

Reviews

From the reviews:

"Within this book's 16 chapters, readers will find a rich source of not only step-by-step protocols and useful lists of the chemicals, reagents, and equipment needed to perform them, but also useful troubleshooting tips and experimental notes that can assist in their successful execution. ... the book is a treasure of data, facts, protocols, and references that nearly every cell biologist and biochemist with an interest in molecular motors will cherish." (Tzvi Tzfira, The Quarterly Review of Biology, Vol. 83, 2008)


Table of contents (16 chapters)

Table of contents (16 chapters)
  • Equilibrium Binding of Proteins to F-Actin

    Pages 1-22

    Chalovich, Joseph M.

  • Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin Using Co-Sedimentation and ATPase Assays

    Pages 23-36

    Reddy, Anireddy S. N.

  • In Vitro and In Vivo Analysis of Microtubule-Destabilizing Kinesins

    Pages 37-49

    Stumpff, Jason (et al.)

  • Approaches to Kinesin-1 Phosphorylation

    Pages 51-69

    Morfini, Gerardo (et al.)

  • Protein Modification to Probe Intradynein Interactions and In Vivo Redox State

    Pages 71-83

    Wakabayashi, Ken-ichi (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-59745-490-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-58829-665-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-61737-706-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Molecular Motors
Book Subtitle
Methods and Protocols
Editors
  • Ann O. Sperry
Series Title
Methods in Molecular Biology
Series Volume
392
Copyright
2007
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-490-2
DOI
10.1007/978-1-59745-490-2
Hardcover ISBN
978-1-58829-665-8
Softcover ISBN
978-1-61737-706-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 252
Number of Illustrations
37 b/w illustrations
Topics