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  • © 2007

Proteomics of Human Body Fluids

Principles, Methods, and Applications

  • The first collection of applications of proteomics to analyze various human body fluids
  • Provides basic principles and strategies for proteomic analysis of human body fluids
  • Offers more details regarding methodologies and recent findings

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Table of contents (24 chapters)

  1. Front Matter

    Pages i-xiii
  2. Principles of Proteomics Applied to Human Body Fluids

    1. Front Matter

      Pages 1-1
    2. Proteomic Strategies for Analyzing Body Fluids

      • Sung-Min Ahn, Richard J. Simpson
      Pages 3-30
    3. Sample Preparation of Body Fluids for Proteomics Analysis

      • Natalia Govorukhina, Rainer Bischoff
      Pages 31-69
    4. Multiplexed Immunoassays for Protein Profiling in Human Body Fluids

      • Silke Wittemann, Dominic P. Eisinger, Laurie L. Stephen, Thomas O. Joos
      Pages 71-82
    5. Modification-Specific Proteomic Analysis of Glycoproteins in Human Body Fluids by Mass Spectrometry

      • Jakob Bunkenborg, Per Hägglund, Ole Nørregaard Jensen
      Pages 107-128
    6. Plasma Proteome Database

      • Malabika Sarker, G. Hanumanthu, Akhilesh Pandey
      Pages 129-136
    7. 2D PAGE Databases for Proteins in Human Body Fluids

      • Christine Hoogland, Khaled Mostaguir, Jean-Charles Sanchez, Denis F. Hochstrasser, Ron D. Appel
      Pages 137-146
    8. Bioinformatics and Experimental Design for Biomarker Discovery

      • Marc R. Wilkins, Sybille M. N. Hunt
      Pages 147-174
  3. Proteomic Analysis of Specific Types of Human Body Fluids

    1. Front Matter

      Pages 193-193
    2. Methods, Findings, applications, Perspectives, and Future Directions

      1. The Human Plasma and Serum Proteome
        • Gilbert S. Omenn, Rajasree Menon, Marcin Adamski, Thomas Blackwell, Brian B. Haab, Weimin Gao et al.
        Pages 195-224
      2. Proteomics of Human Urine
        • Visith Thongboonkerd, Pedro R. Cutillas, Robert J. Unwin, Stefan Schaub, Peter Nickerson, Marion Haubitz et al.
        Pages 225-268
      3. Proteomics of Human Cerebrospinal Fluid
        • Margareta Ramström, Jonas Bergquist
        Pages 269-284
      4. Proteomics of Pleural Effusion
        • Joost Hegmans, Annabrita Hemmes, Bart Lambrecht
        Pages 285-307
      5. Proteomics of Bronchoalveolar Lavage Fluid and Sputum
        • Ruddy Wattiez, Olivier Michel, Paul Falmagne
        Pages 309-325
      6. Proteomics of Sinusitis Nasal Lavage Fluid
        • Begona Casado, Simona Viglio, James N. Baraniuk
        Pages 327-346
      7. Proteomics of Human Saliva
        • Francisco M. L. Amado, Rui M. P. Vitorino, Maria J. C. Lobo, Pedro M. D. N. Domingues
        Pages 347-376
      8. Proteomics of Human Pancreatic Juice
        • Mads Grønborg, Anirban Maitra, Akhilesh Pandey
        Pages 377-397

About this book

Rapid growth of the “proteomics” field during the past twelve years has c- tributed to significant advances in science. To date, proteomic technologies have been widely applied to examining various kinds of biological materials. Clinical proteomics is the concept of using proteomic techniques to evaluate the proteomes in clinical samples for biomarker discovery and for better understa- ing of normal physiology and pathogenic mechanisms of human diseases. Tra- lating the proteomic information to clinical practice may lead to the ultimate goals of earlier diagnosis, improved therapeutic outcome and successful prev- tion of diseases. Human body fluids are produced, secreted, and/or excreted from various tissues or organs. Major compositions of body fluids are water, organic s- stances and inorganic compounds. These compositions vary in each body fluid, making one’s function different from the others. Analyses of protein com- nents in individual fluids would increase current knowledge on the biology and physiology of various organ systems, and on the pathophysiology of diseases, which cause alterations in protein production, secretion, and/or excretion from the affected tissues or organs into the body fluids. Additionally, human body fluids are the main targets and valuable sources for biomarker discovery. As the high-throughput capability and applicability of proteomics for analyzing proteins in the body fluids have been already proven, these desires are most likely achievable by using proteomic technologies.

Editors and Affiliations

  • Medical Molecular Biology Unit Office for Research and Development Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

    Visith Thongboonkerd

Bibliographic Information

  • Book Title: Proteomics of Human Body Fluids

  • Book Subtitle: Principles, Methods, and Applications

  • Editors: Visith Thongboonkerd

  • DOI: https://doi.org/10.1007/978-1-59745-432-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-657-3Published: 25 September 2007

  • Softcover ISBN: 978-1-61737-700-6Published: 10 December 2010

  • eBook ISBN: 978-1-59745-432-2Published: 23 January 2008

  • Edition Number: 1

  • Number of Pages: XIII, 533

  • Number of Illustrations: 96 b/w illustrations, 7 illustrations in colour

  • Topics: Biochemistry, general, Cell Biology, Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access