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Methods in Molecular Medicine

Microtubule Protocols

Editors: Zhou, Jun (Ed.)

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  • Includes methods for the development and discovery of novel agents that target microtubules
  • Includes readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes
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About this book

Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Microtubules

    Pages 1-16

    Wade, Richard H., PhD

  • Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure

    Pages 17-28

    Andreu, José Manuel, PhD

  • Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts

    Pages 29-43

    Gache, Vincent, PhD (et al.)

  • Preparation and Characterization of Posttranslationally Modified Tubulins From Artemia franciscana

    Pages 45-63

    O’Connell, Paul A., MSc (et al.)

  • Studying the Structure of Microtubules by Electron Microscopy

    Pages 65-91

    Amos, Linda A., PhD (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-442-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-58829-642-9
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-691-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Microtubule Protocols
Editors
  • Jun Zhou
Series Title
Methods in Molecular Medicine
Series Volume
137
Copyright
2007
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-442-1
DOI
10.1007/978-1-59745-442-1
Hardcover ISBN
978-1-58829-642-9
Softcover ISBN
978-1-61737-691-7
Series ISSN
1543-1894
Edition Number
1
Number of Pages
X, 305
Topics

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