Methods in Molecular Biology

Plant Proteomics

Methods and Protocols

Editors: Thiellement, Hervé (Ed.)

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About this book

The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).

Table of contents (29 chapters)

  • Total Protein Extraction with TCA-Acetone

    Méchin, Valérie (et al.)

    Pages 1-8

  • Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues

    Faurobert, Mireille (et al.)

    Pages 9-14

  • Protein Extraction from Cereal Seeds

    Branlard, Gérard (et al.)

    Pages 15-25

  • Protein Extraction from Xylem and Phloem Sap

    Kehr, Julia (et al.)

    Pages 27-35

  • Protein Extraction from Woody Plants

    Plomion, Christophe (et al.)

    Pages 37-41

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59745-227-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-635-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • ISBN 978-1-61737-686-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Hervé Thiellement
Series Title
Methods in Molecular Biology
Series Volume
355
Copyright
2007
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-227-4
DOI
10.1385/1597452270
Hardcover ISBN
978-1-58829-635-1
Softcover ISBN
978-1-61737-686-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
416
Number of Illustrations and Tables
135 b/w illustrations
Topics