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Methods in Molecular Biology

MicroRNA Protocols

Editors: Ying, Shao-Yao (Ed.)

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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59745-123-9
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-581-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $229.00
price for USA in USD
  • ISBN 978-1-61737-656-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and assaying it. The authors provide diverse, novel, and useful descriptions of miRNAs in several species-including plants, worms, flies, fish, chicks, mice, and humans-and show how they have been employed to develop miRNA as a potential drug design tool. Highlights include miRNA and their target prediction by computer analysis, functional assays of miRNA in vitro and in vivo, and transgenic animal models using miRNA technologies. Additional chapters cover gene regulation, small RNA function, RNA interference mechanisms, and transgenetics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Eminently practical and cutting-edge, MicroRNA Protocols affords biomedical researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease and design new therapeutic strategies.

Table of contents (26 chapters)

  • The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions

    Ying, Shao-Yao (et al.)

    Pages 1-18

  • Structure Analysis of MicroRNA Precursors

    Krol, Jacek (et al.)

    Pages 19-32

  • MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex

    Gregory, Richard I. (et al.)

    Pages 33-47

  • Recognition and Cleavage of Primary MicroRNA Transcripts

    Zeng, Yan (et al.)

    Pages 49-56

  • Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery

    Muljo, Stefan A. (et al.)

    Pages 57-72

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59745-123-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-581-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $229.00
price for USA in USD
  • ISBN 978-1-61737-656-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
MicroRNA Protocols
Editors
  • Shao-Yao Ying
Series Title
Methods in Molecular Biology
Series Volume
342
Copyright
2006
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-123-9
DOI
10.1385/1597451231
Hardcover ISBN
978-1-58829-581-1
Softcover ISBN
978-1-61737-656-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 366
Number of Illustrations and Tables
80 b/w illustrations, 3 illustrations in colour
Topics