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MicroRNA Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Shao-Yao Ying

    1. Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, USA

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Part of the book series: Methods in Molecular Biology (MIMB, volume 342)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions

    • Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, Shi-Lung Lin
    Pages 1-18

  3. Structure Analysis of MicroRNA Precursors

    • Jacek Krol, Wlodzimierz J. Krzyzosiak
    Pages 19-32

  4. MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex

    • Richard I. Gregory, Thimmaiah P. Chendrimada, Ramin Shiekhattar
    Pages 33-47

  5. Recognition and Cleavage of Primary MicroRNA Transcripts

    • Yan Zeng, Bryan R. Cullen
    Pages 49-56

  6. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery

    • Stefan A. Muljo, Chryssa Kanellopoulou
    Pages 57-72

  7. MicroRNAs and Messenger RNA Turnover

    • Julia A. Chekanova, Dmitry A. Belostotsky
    Pages 73-85

  8. Prediction of MicroRNA Targets

    • Marc Rehmsmeier
    Pages 87-99

  9. Prediction of Human MicroRNA Targets

    • Bino John, Chris Sander, Debora S. Marks
    Pages 101-113

  10. Complications in Mammalian MicroRNA Target Prediction

    • Neil R. Smalheiser, Vetle I. Torvik
    Pages 115-127

  11. miRBase:The MicroRNA Sequence Database

    • Sam Griffiths-Jones
    Pages 129-138

  12. Methodologies for High-Throughput Expression Profiling of MicroRNAs

    • Paz Einat
    Pages 139-157

  13. In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development

    • Catherine Kidner, Marja Timmermans
    Pages 159-179

  14. Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA

    • Fengfeng Zhuang, Yi-Hsin Liu
    Pages 181-187

  15. Cloning MicroRNAs From Mammalian Tissues

    • Michael Z. Michael
    Pages 189-207

  16. Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation

    • Hyeyoung Min, Chang-Zheng Chen
    Pages 209-227

  17. Identifying MicroRNA Regulators of Cell Death in Drosophila

    • Chun-Hong Chen, Ming Guo, Bruce A. Hay
    Pages 229-240

  18. MicroRNAs in Human Immunodeficiency Virus-1 Infection

    • Yamina Bennasser, Shu-Yun Le, Man Lung Yeung, Kuan-Teh Jeang
    Pages 241-253

  19. Cloning and Detection of HIV-1-Encoded MicroRNA

    • Shinya Omoto, Yoichi R. Fujii
    Pages 255-265

  20. Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein

    • Ranhui Duan, Peng Jin
    Pages 267-276
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Keywords

About this book

MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.

Editors and Affiliations

  • Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, USA

    Shao-Yao Ying

Bibliographic Information

  • Book Title: MicroRNA Protocols

  • Editors: Shao-Yao Ying

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597451231

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-581-1Published: 01 April 2006

  • Softcover ISBN: 978-1-61737-656-6Published: 01 December 2010

  • eBook ISBN: 978-1-59745-123-9Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 366

  • Number of Illustrations: 80 b/w illustrations, 3 illustrations in colour

  • Topics: Cell Biology

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