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Methods in Molecular Biology

Quantitative Proteomics by Mass Spectrometry

Editors: Sechi, Salvatore (Ed.)

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  • Cutting-edge techniques for introducing isotopic labels
  • Affinity enrichment of phosphopeptides coupled with stable-isotope labeling
  • Tandem MS for disease detection
  • Procedures for characterization of the phosphoproteome
  • Quantification of proteins by MS without isotopic labels
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eBook $129.00
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  • ISBN 978-1-59745-255-7
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Hardcover $169.99
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Softcover $169.99
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About this book

Quantitative Proteomics by Mass Spectrometry, from the Methods in Molecular Biology™ series, is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers.

Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS..


Reviews

From the reviews:

“Quantitative Proteomics by Mass Spectrometry describes protocols of various quantitative proteomics procedures utilizing mass spectrometry. … The publication of this book is timely. … provides useful information regarding sample preparation/handling, reagent preparation/handling, mass spectrometry analysis etc. for successful experiments. … In summary, the book serves as an excellent guide for both the experienced investigator and novice in the field who is contemplating using quantitative proteomics by mass spectrometry.”­­­ (Masaru Miyagi, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)

Table of contents (14 chapters)

Table of contents (14 chapters)
  • Acrylamide—A Cysteine Alkylating Reagent for Quantitative Proteomics

    Pages 1-16

    Turko, Illarion V. (et al.)

  • Using Stable Isotope Tagging and Mass Spectrometry to Characterize Protein Complexes and to Detect Changes in Their Composition

    Pages 17-35

    Ranish, Jeffrey A. (et al.)

  • Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics

    Pages 37-52

    Ong, Shao-En (et al.)

  • Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope Labeling

    Pages 53-70

    Sato, Toshitaka (et al.)

  • The Absolute Quantification Strategy

    Pages 71-86

    Gerber, Scott A. (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-59745-255-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Quantitative Proteomics by Mass Spectrometry
Editors
  • Salvatore Sechi
Series Title
Methods in Molecular Biology
Series Volume
359
Copyright
2007
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-255-7
DOI
10.1007/978-1-59745-255-7
Hardcover ISBN
978-1-58829-571-2
Softcover ISBN
978-1-61737-648-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 218
Number of Illustrations
48 b/w illustrations, 4 illustrations in colour
Topics