Methods in Biotechnology

Microbial Processes and Products

Editors: Barredo, José-Luis (Ed.)

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About this book

The development of recombinant DNA techniques over the last 20 years has greatly expanded the opportunities for using microorganisms to produce a broad range of valuable substances. In Microbial Processes and Products, outstanding leaders in using microorganisms as cell factories describe in detail their best laboratory procedures for many processes and products mediated by microorganisms. An overview chapter describes how to develop strain improvement programs and strategies to optimize fermentation processes. Taking advantage of the most recent developments in such processes, the authors offer step-by-step experimental methods for the optimal design of microbial metabolite production, including semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination, L-lysine, vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids b-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation. Additionally, the use of phenylacetyl-CoA catabolon for enzymatic synthesis of penicillins, aromatic biotransformations, synthesis of new bioplastics, biosensor design, or synthesis of drug vehicles, and the development of a phosphate encoding gene as a reporter and to monitor gene expression are illustrated.
The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection. Additional chapters offer techniques for analysis of antimicrobial metabolites and carotenoids, volatile sulfur compounds, metabolic pathway fluxes, gene expression arrays, proteome analysis, bacterial modulation of the innate immune response, bioleaching activity, and heavy metal remediation. Finally, three overview chapters on transport of biological material, deposit of biological material for patent purposes, and protection of biotechnological inventions are shown. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Enzymes and Biotransformations, describes in detail cutting-edge techniques for the screening, evolution, production, immobilization, and use of enzymes.
Wide-ranging and practical, Microbial Processes and Products offers laboratory and industrial scientists a wealth of readily reproducible techniques for the successful microbial generation of biochemical products to serve the needs of human health, nutrition, and environmental protection.

Reviews

Microbial Processes and Products is the latest in the series Methods in Biotechnology™, some issues of which have been highly focused and widely relevant (such as Enzymes in Nonaqueous Solvents, Vulfson et al., 2001). This volume (and indeed the entire series) is targeted to the research community. Those researchers finding a relevant chapter in this text are likely to be rewarded. As the series title suggests, this is a 'methods' volume and the sections are packed with detailed protocols, tips and useful practical advice. The text is generally readable and the chapters are well referenced. -Don Cowan, University of the Western Cape

"...the sections are packed with detailed protocols, tips, and useful practical advice." - Microbiology Today

Table of contents (28 chapters)

Table of contents (28 chapters)
  • Development of Improved Strains and Optimization of Fermentation Processes

    Pages 1-23

    Han, Lei (et al.)

  • Experimental Design in Microbiology

    Pages 25-39

    Vanot, Guillaume E. (et al.)

  • Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acid

    Pages 41-64

    RodrÍguez-Sáiz, Marta (et al.)

  • Production of Erythromycin With Saccharopolyspora erythraea

    Pages 65-90

    Minas, Wolfgang

  • The Phenylacetyl-CoA Catabolon

    Pages 91-129

    Luengo, José M. (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-59259-847-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $219.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Microbial Processes and Products
Editors
  • José-Luis Barredo
Series Title
Methods in Biotechnology
Series Volume
18
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-847-2
DOI
10.1385/1592598471
Hardcover ISBN
978-1-58829-548-4
Softcover ISBN
978-1-61737-635-1
Series ISSN
1940-6061
Edition Number
1
Number of Pages
XIV, 516
Topics