Methods in Molecular Biology

Transmembrane Signaling Protocols

Editors: Ali, Hydar, Bodduluri, Haribabu (Eds.)

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About this book

The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today.

Reviews

From Reviews of the First Edition...

"...well put together...covers a range of signaling protocols and would serve well as a reference for what methods are currently being used in the field." -Molecular Biotechnology

"...a good book for a non-specialist entering the field and specialists looking for well-described techniques." -Microbiology Today

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Transmembrane Signaling by G Protein-Coupled Receptors

    Luttrell, Louis M.

    Pages 3-49

  • Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases

    Natarajan, Kanchana (et al.)

    Pages 51-77

  • Protein-Protein Interactions in Signaling Cascades

    Mayer, Bruce J.

    Pages 79-99

  • Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells

    Majka, Marcin (et al.)

    Pages 103-114

  • Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4

    Wang, Zi-xuan (et al.)

    Pages 115-127

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59745-048-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-58829-546-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-633-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Transmembrane Signaling Protocols
Editors
  • Hydar Ali
  • Haribabu Bodduluri
Series Title
Methods in Molecular Biology
Series Volume
332
Copyright
2006
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-048-5
DOI
10.1385/1597450480
Hardcover ISBN
978-1-58829-546-0
Softcover ISBN
978-1-61737-633-7
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 364
Number of Illustrations
53 b/w illustrations, 1 illustrations in colour
Topics