Methods in Molecular Medicine

Fibrosis Research

Methods and Protocols

Editors: Varga, John, Brenner, David A., Phan, Sem H. (Eds.)

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eBook $149.00
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  • ISBN 978-1-59259-940-0
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Hardcover $199.99
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Softcover $199.99
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  • ISBN 978-1-61737-592-7
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About this book

Fibrosis, or scar tissue, is increasingly recognized as an important feature of many chronic diseases of the lung, kidney, heart, skin, eye, and bone. With some 45% of deaths in the United States attributed to fibrosing disorders and the lack of any effective treatment, research into the cellular, molecular, and genetic basis of fibrosis has exploded. In Fibrosis Research: Methods and Protocols, leading investigators at the forefront of this emerging field review the highlights of current fibrosis work in a variety of disciplines and the experimental methodologies used to uncover the mechanisms that drive it. In their discussion of research techniques utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. Additional techniques presented cover the evolving genetic methods for identifying "fibrosis genes" or allelic polymorphisms in human populations, microarray studies for describing global patterns of gene expression associated with fibrosis, and proteomic approaches to the same. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and state-of-the-art, Fibrosis Research: Methods and Protocols offers investigators all the powerful experimental methodologies, techniques, and strategies needed today to study and ultimately understand the daunting problem of pathological fibrosis.

Reviews

"...a must for the shelf of every researcher and physician interested in fibrotic diseases." -Rheumatology

Table of contents (22 chapters)

  • Pulmonary Fibrosis

    Zisman, David A. (et al.)

    Pages 3-44

  • Renal Fibrosis

    Schnaper, H. William

    Pages 45-68

  • Transforming Growth Factor-β

    Mauviel, Alain

    Pages 69-80

  • Isolation and Culture of Skin Fibroblasts

    Rittié, Laure (et al.)

    Pages 83-98

  • Isolation and Culture of Hepatic Stellate Cells

    Weiskirchen, Ralf (et al.)

    Pages 99-113

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59259-940-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-479-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • ISBN 978-1-61737-592-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fibrosis Research
Book Subtitle
Methods and Protocols
Editors
  • John Varga
  • David A. Brenner
  • Sem H. Phan
Series Title
Methods in Molecular Medicine
Series Volume
117
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-940-0
DOI
10.1385/1592599400
Hardcover ISBN
978-1-58829-479-1
Softcover ISBN
978-1-61737-592-7
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XVI, 392
Number of Illustrations and Tables
80 b/w illustrations
Topics