Receptor Binding Techniques

1. Front Matter

   Pages i-x

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2. Receptor Databases and Computational Websites for Ligand Binding

   • Brinda K. Rana, Paul A. Insel

   Pages 1-15

3. Identification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR® Assays

   • Nicola M. Robas, Mark D. Fidock

   Pages 17-26

4. Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR

   • Stephen A. Douglas, Zhaohui Ao, Douglas G. Johns, Kristeen Maniscalco, Robert N. Willette, Lea Sarov-Blat et al.

   Pages 27-49

5. mRNA

   • Alessandra P. Princivalle, Rachel M. C. Parker, Terri J. Dover, Nicholas M. Barnes

   Pages 51-92

6. Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established and Emerging Orphan Receptor Systems

   • Anthony P. Davenport, Rhoda E. Kuc

   Pages 93-120

7. Measurement of Radioligand Binding by Scintillation Proximity Assay

   • Jenny Berry, Molly Price-Jones

   Pages 121-138

8. Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels

   • David A. Walsh, John Wharton

   Pages 139-154

9. Immunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application to the Phenotypic Characterization of Knock-Out Mice

   • Anthony P. Davenport, Rhoda E. Kuc

   Pages 155-172

10. Live Cell Imaging of G Protein-Coupled Receptors

    • Burkhard Wiesner, Michael Beyermann, Alexander Oksche

    Pages 173-202

11. Imaging and Characterization of Radioligands for Positron Emission Tomography Using Quantitative Phosphor Imaging Autoradiography

    • Peter JohnstrÖm, Anthony P. Davenport

    Pages 203-216

12. Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography

    • Peter Johnström, Tim D. Fryer, Hugh K. Richards, Olivier Barret, Anthony P. Davenport

    Pages 217-246

13. Back Matter

    Pages 233-240

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A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.

• PCR
• cell
• enzymes
• hybridization
• ion channels
• lipide
• microscopy
• protein
• radiography
• research

From Reviews of the First Edition...

"...a very useful and practical addition to the field of receptor pharmacology...should be on the bench of every student of receptor pharmacology..." -Doody's Health Sciences Book Review Journal

• Clinical Pharmacology Unit, University of Cambridge, Centre for Clinical Investigation, Addenbrooke7s Hospital, Cambridge, UK

  Anthony P. Davenport

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