Methods in Molecular Biology

Receptor Binding Techniques

Editors: Davenport, Anthony P. (Ed.)

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About this book

Maintaining the high standards set by the successful first edition, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical industry and academia take advantage of the latest developments to provide detailed practical methods for studying receptors in silico, in vitro, and in vivo. These readily reproducible techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knock-out mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan polymerase chain reaction (PCR). These ligand binding techniques are perfect for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state of the art, Receptor Binding Techniques, Second Edition offers academic and commercial researchers in the pharmaceutical and biotechnology industries a set of proven techniques for the successful characterization of receptors and the phenotyping of transgenic animals, including knock-outs.

Reviews

From Reviews of the First Edition...

"...a very useful and practical addition to the field of receptor pharmacology...should be on the bench of every student of receptor pharmacology..." -Doody's Health Sciences Book Review Journal

Table of contents (11 chapters)

Table of contents (11 chapters)
  • Receptor Databases and Computational Websites for Ligand Binding

    Pages 1-15

    Rana, Brinda K. (et al.)

  • Identification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR® Assays

    Pages 17-26

    Robas, Nicola M. (et al.)

  • Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR

    Pages 27-49

    Douglas, Stephen A. (et al.)

  • mRNA

    Pages 51-92

    Princivalle, Alessandra P. (et al.)

  • Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established and Emerging Orphan Receptor Systems

    Pages 93-120

    Davenport, Anthony P. (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-59259-927-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts from 10 eBooks
Hardcover $159.99
price for USA in USD
Softcover $159.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Receptor Binding Techniques
Editors
  • Anthony P. Davenport
Series Title
Methods in Molecular Biology
Series Volume
306
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-927-1
DOI
10.1385/1592599273
Hardcover ISBN
978-1-58829-420-3
Softcover ISBN
978-1-61737-556-9
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 240
Number of Illustrations
71 b/w illustrations
Topics