Methods in Molecular Biology

RNA-Protein Interaction Protocols

Editors: Lin, Ren-Jang (Ed.)

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  • Includes various methods for affinity-purifying cellular or in vitro reconstituted RNPs
  • A collection of methods for studying RNA-protein interaction that has occurred inside a cell
  • Contains various methods for assaying protein footprints on RNA
  • Includes a number of methods for mapping protein regions involved in RNA interaction
  • Other new techniques explained, including quantifying specific RNAs, designing siRNAs, measuring RNPase activity, etc.
  • Example methods in many cellular events
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eBook $129.00
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  • ISBN 978-1-60327-475-3
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Hardcover $169.99
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Softcover $169.99
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About this book

Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership.

Reviews

From the reviews of the second edition:

"Detailed protocols for analysis of specific examples of RNA protein interactions are described. … Anyone studying RNA protein interactions should seriously consider reading this book, not only as a technical reference, but also as a source of ideas. … It’s a book for the lab bench and the PIs office. Highly recommended." (Laurence Tiley, Microbiology Today, February, 2009)

"The second edition of RNA-protein interaction protocols is part of the Methods in Molecular Biology series and includes detailed methods covering a wide range of approaches to characterise RNA-protein interactions. … This book … is well written, well indexed and well referenced. Each protocol is described in enough detail to be followed by an experienced user. For an RNA-based laboratory this book provides an excellent description of the latest techniques with particular attention given to manipulation of RNPs from in vivo sources." (Matthew Bentham, British Toxicology Society Newsletter, June-August, 2009)


Table of contents (25 chapters)

Table of contents (25 chapters)
  • Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RNA Affinity Chromatography

    Pages 1-8

    Sharma, Shalini

  • An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase

    Pages 9-22

    Kurth, Isabel (et al.)

  • RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNA–Protein Complexes

    Pages 23-40

    Walker, Scott C. (et al.)

  • Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA

    Pages 41-63

    Dery, Kenneth J. (et al.)

  • Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques

    Pages 65-84

    Stevens, Scott W.

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-60327-475-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
RNA-Protein Interaction Protocols
Editors
  • Ren-Jang Lin
Series Title
Methods in Molecular Biology
Series Volume
488
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-475-3
DOI
10.1007/978-1-60327-475-3
Hardcover ISBN
978-1-58829-419-7
Softcover ISBN
978-1-61737-555-2
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 416
Topics