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Human Papillomaviruses

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Clare Davy
    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. John Doorbar

    1. Division of Virology, The National Institute for Medical Research, Mill Hill, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 119)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Identification of New Papillomavirus Types

    • Ethel-Michele de Villiers, Corinna Whitley, Karin Gunst
    Pages 1-13

  3. Identification of HPV Variants

    • John Cason, Jon Bible, Christine Mant
    Pages 15-25

  4. Histochemical Analysis of Cutaneous HPV-Associated Lesions

    • Kiyofumi Egawa
    Pages 27-40

  5. Histological Analysis of Cervical Intraepithelial Neoplasia

    • Michael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, David Jenkins
    Pages 41-48

  6. Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections

    • Woei Ling Peh, John Doorbar
    Pages 49-59

  7. Detection and Quantitation of HPV Gene Expression Using Real-Time PCR

    • Rashmi Seth, John Rippin, Li Guo, David Jenkins
    Pages 61-72

  8. Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers

    • Svetlana Vinokurova, Nicolas Wentzensen, Magnus von Knebel Doeberitz
    Pages 73-83

  9. Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN

    • Jan P. A. Baak, Arnold-Jan Kruse
    Pages 85-99

  10. HPV DNA Detection and Typing in Cervical Scrapes

    • Peter J. F. Snijders, Adriaan J. C. van den Brule, Marcel V. Jacobs, René P. Pol, Chris J. L. M. Meijer
    Pages 101-114

  11. HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions

    • Maurits de Koning, Linda Struijk, Mariet Feltkamp, Jan ter Schegget
    Pages 115-127

  12. Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies

    • Margaret Anne Stanley
    Pages 129-139

  13. Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic “Raft” Cultures

    • Paul F. Lambert, Michelle A. Ozbun, Asha Collins, Sigrid Holmgren, Denis Lee, Tomomi Nakahara
    Pages 141-155

  14. Differentiation of HPV-Containing Cells Using Organotypic “Raft” Culture or Methylcellulose

    • Regina Wilson, Laimonis A. Laimins
    Pages 157-169

  15. Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture

    • Margaret E. McLaughlin-Drubin, Craig Meyers
    Pages 171-186

  16. Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic “Raft” Cultures

    • N. Sanjib Banerjee, Louise T. Chow, Thomas R. Broker
    Pages 187-202

  17. The HPV Xenograft Severe Combined Immunodeficiency Mouse Model

    • William Bonnez
    Pages 203-216

  18. The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease

    • Janet L. Brandsma
    Pages 217-235

  19. Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology

    • Yong Liu, Hong You, Paul L. Hermonat
    Pages 237-245

  20. Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae

    • Peter C. Angeletti
    Pages 247-260
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Keywords

About this book

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • Division of Virology, The National Institute for Medical Research, Mill Hill, London, UK

    John Doorbar

Bibliographic Information

  • Book Title: Human Papillomaviruses

  • Book Subtitle: Methods and Protocols

  • Editors: Clare Davy, John Doorbar

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592599826

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-373-2Published: 01 September 2005

  • Softcover ISBN: 978-1-61737-526-2Published: 10 November 2010

  • eBook ISBN: 978-1-59259-982-0Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVI, 496

  • Number of Illustrations: 153 b/w illustrations

  • Topics: Medical Microbiology, Infectious Diseases

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