- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.
- Table of contents (18 chapters)
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Reflecting on Confocal Microscopy: A Personal Perspective
Pages 1-7
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Laser Scanning Confocal Microscopy: History, Applications, and Related Optical Sectioning Techniques
Pages 9-47
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Confocal Microscopy on the Internet
Pages 49-96
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Using Photoshop with Images Created by a Confocal System
Pages 97-128
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Clearing Up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy
Pages 129-148
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Table of contents (18 chapters)
- Download Preface 1 PDF (44.2 KB)
- Download Sample pages 1 PDF (2 MB)
- Download Table of contents PDF (46.1 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Confocal Microscopy
- Book Subtitle
- Methods and Protocols
- Editors
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- Stephen W. Paddock
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1075
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-60761-847-8
- DOI
- 10.1007/978-1-60761-847-8
- Hardcover ISBN
- 978-1-58829-351-0
- Softcover ISBN
- 978-1-4939-5067-6
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XI, 381
- Number of Illustrations
- 79 b/w illustrations, 51 illustrations in colour
- Topics
