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Methods in Molecular Biology

Phosphodiesterase Methods and Protocols

Editors: Lugnier, Claire (Ed.)

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About this book

The multigenic family of cyclic nucleotide phosphodiesterases (PDEs) is now known to offer many new therapeutic possibilities through their influence over intracellular signaling, though their precise cell mechanisms to modulating that process remain uncertain. In Phosphodiesterase Methods and Protocols, a panel of research leaders in the PDE field describes new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. Presented in step-by-step detail, these readily reproducible methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatiotemporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creating specific tools and drugs. Additional chapters discuss the generation of PDE4 knockout mice to demonstrate not only the potential role of targeted PDEs, but also their use in further studies of the central role of PDE regulation in intracellular signaling control. These techniques offer clinicians a way to find new therapies for numerous pathologies whose molecular origins are unknown and whose treatment is symptomatic. Alterations of intracellular signaling related to PDE deregulation in such pathologies as inflammation, neurodegeneration, and cancer may explain the difficulties observed in their prevention and treatment. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Path-breaking and highly practical, Phosphodiesterase Methods and Protocols offers cell biologists, molecular biologists, pharmacologists, and medicinal chemists a broad ranging survey of the advanced tools and concepts needed to understand the role of PDEs in physiological functions, their implications in several critical pathologies, and the opportunities they offer for new drug design.

Reviews

"...interesting and thought provoking, but technically challenging...excellent guide to current research techniques in the field of phosphodiesterase." - Doody's Health Sciences Book Review Journal

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Study of Cyclic Adenosine Monophosphate Microdomains in Cells

    Pages 1-13

    Mongillo, Marco (et al.)

  • High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains

    Pages 15-26

    Karpen, Jeffrey W. (et al.)

  • Cygnets

    Pages 27-43

    Honda, Akira (et al.)

  • High-Throughput Screening of Phosphodiesterase Activity in Living Cells

    Pages 45-61

    Rich, Thomas C. (et al.)

  • Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts

    Pages 63-74

    Keravis, Thérèse (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59259-839-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-58829-314-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-487-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Phosphodiesterase Methods and Protocols
Editors
  • Claire Lugnier
Series Title
Methods in Molecular Biology
Series Volume
307
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-839-7
DOI
10.1385/1592598390
Hardcover ISBN
978-1-58829-314-5
Softcover ISBN
978-1-61737-487-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
336
Number of Illustrations
92 b/w illustrations, 1 illustrations in colour
Topics