Methods in Molecular Biology

Nuclear Transfer Protocols

Cell Reprogramming and Transgenesis

Editors: Verma, Paul J. (Ed.)

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  • ISBN 978-1-59745-154-3
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About this book

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.

Reviews

"...an essential comprehensive handbook for both research and commercials labs involved in nuclear transfer, and will be of interest to molecular and stem cell biologists and biotechnologists involved with reprogramming, nuclear transfer and transgensis."  -Biotech-online

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Origin and Progress of Nuclear Transfer in Nonmammalian Animals

    Pages 3-31

    Berardino, Marie A.

  • In Vitro Maturation and Embryo Production in Cattle

    Pages 35-42

    Sirard, Marc-André (et al.)

  • Activation of Oocytes After Nuclear Transfer

    Pages 43-58

    Macháty, Zoltán

  • Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos

    Pages 59-77

    Heindryckx, Björn (et al.)

  • Maintenance of Pregnancy in Pigs With Limited Viable Embryos

    Pages 79-90

    King, Tim (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-154-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
  • Institutional customers should get in touch with their account manager
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Nuclear Transfer Protocols
Book Subtitle
Cell Reprogramming and Transgenesis
Editors
  • Paul J. Verma
Series Title
Methods in Molecular Biology
Series Volume
348
Copyright
2006
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-154-3
DOI
10.1007/978-1-59745-154-3
Hardcover ISBN
978-1-58829-280-3
Softcover ISBN
978-1-61737-468-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 338
Topics