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Methods in Molecular Biology

Immunochemical Protocols

Editors: Burns, Robert (Ed.)

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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-873-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-58829-274-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-466-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes.

Reviews

Reviews of Previous editions:
"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."
-Microbiology Today

An excellent and well arranged compilation of a range of antibody techniques....An excellent laboratory manual.-Immunoassay

Table of contents (22 chapters)

  • Immunization Strategies for Antibody Production

    Burns, Robert

    Pages 1-11

  • Synthetic Peptides as Antigens for Antibody Production

    Hancock, David C. (et al.)

    Pages 13-25

  • Production of Polyclonal Antibodies in Rabbits

    Hancock, David C. (et al.)

    Pages 27-39

  • Making Hybridomas

    Burns, Robert

    Pages 41-53

  • Growing Hybridomas

    Entrican, Gary (et al.)

    Pages 55-70

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-873-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-58829-274-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-466-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Immunochemical Protocols
Editors
  • Robert Burns
Series Title
Methods in Molecular Biology
Series Volume
295
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-873-1
DOI
10.1385/1592598730
Hardcover ISBN
978-1-58829-274-2
Softcover ISBN
978-1-61737-466-1
Series ISSN
1064-3745
Edition Number
3
Number of Pages
X, 318
Number of Illustrations
22 b/w illustrations
Topics