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Methods in Molecular Biology

Germ Cell Protocols

Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning

Editors: Schatten, Heide (Ed.)

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About this book

Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, the imaging of cell structures, and the cryopreservation of germ cells. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. There are also methods for PCR-based cloning, differentiating embryonic stem cells into embryonic bodies, live imaging of mammalian embryos using multiphoton microscopy, and the cloning of pig, cattle, rabbit, and mouse oocytes. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying earlier volume, Sperm and Oocyte Analysis, focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques.
Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos

    Pages 1-34

    Boiani, Michele (et al.)

  • Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans

    Pages 35-48

    Jedrusik, Monika A. (et al.)

  • cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development

    Pages 49-65

    Hemberger, Myriam

  • PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells

    Pages 67-78

    Venkataraman, Thiagarajan (et al.)

  • Differentiating Embryonic Stem Cells into Embryoid Bodies

    Pages 79-98

    Höpfl, Gisele (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-59259-741-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-58829-257-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-457-9
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Germ Cell Protocols
Book Subtitle
Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning
Editors
  • Heide Schatten
Series Title
Methods in Molecular Biology
Series Volume
254
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-741-3
DOI
10.1385/1592597416
Hardcover ISBN
978-1-58829-257-5
Softcover ISBN
978-1-61737-457-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 418
Topics

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