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Methods in Molecular Biology

RNA Interference, Editing, and Modification

Methods and Protocols

Editors: Gott, Jonatha M. (Ed.)

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  • ISBN 978-1-59259-775-8
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Hardcover $179.99
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Softcover $179.99
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  • ISBN 978-1-61737-445-6
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About this book

The study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed to study these processes in most of the major biological systems in which they are known to occur-a wide range of organisms that includes worms, flies, trypanosomes, mammals, and plants. A historical overview of each subject is provided to put related chapters into a larger context. RNA interference (RNAi) has also emerged in recent years as an important and promising tool for gene discovery and reverse genetic engineering. Application of this cutting-edge technique is described for a range of experimental systems. Topics of interest include stable and transient RNA interference, RNA editing, gene silencing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs, many of which are involved in RNA interference or modification. In addition, techniques used to study RNA editing mechanisms are elaborated for both systems that involve the conversion of one base to another and insertion-deletion editing. To promote the adaptation of their assays and approaches for use in other biological systems, the authors have included a range of methods representative of the major experimental systems in a given field. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, RNA Interference, Editing, and Modification: Methods and Protocols offers not only geneticists, molecular biologists, and bioinformaticists a comprehensive collection of readily reproducible techniques for the elucidation of gene function and the alteration of gene expression, but also describes how best they may be productively used to address outstanding mechanistic questions and adapted to novel biological systems.

Reviews

"...the book delivers detailed experimental protocols that are clearly presented and easy to understand...it delivers practical information...." -ChemBioChem

Table of contents (21 chapters)

  • RNA Interference

    Montgomery, Mary K.

    Pages 3-21

  • Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans

    Hull, Dawn (et al.)

    Pages 23-58

  • Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells

    Caudy, Amy A. (et al.)

    Pages 59-72

  • Analysis of Gene Function in Trypanosoma brucei Using RNA Interference

    Djikeng, Appolinaire (et al.)

    Pages 73-83

  • Short Hairpin Activated Gene Silencing in Mammalian Cells

    Paddison, Patrick J. (et al.)

    Pages 85-100

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-775-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-58829-242-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61737-445-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Interference, Editing, and Modification
Book Subtitle
Methods and Protocols
Editors
  • Jonatha M. Gott
Series Title
Methods in Molecular Biology
Series Volume
265
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-775-8
DOI
10.1385/1592597750
Hardcover ISBN
978-1-58829-242-1
Softcover ISBN
978-1-61737-445-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 436
Topics