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Methods in Molecular Biology

mRNA Processing and Metabolism

Methods and Protocols

Editors: Schoenberg, Daniel R. (Ed.)

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eBook $109.00
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  • ISBN 978-1-59259-750-5
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Hardcover $149.99
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Softcover $149.00
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  • ISBN 978-1-61737-434-0
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About this book

mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.

Reviews

"...the shear diversity of current techniques on offer in this book makes it a valuable resource not only for those studying mRNA processing and metabolism but for those interested in RNA or RNA-protein interactions in general." - ChemBioChem

Table of contents (20 chapters)

  • Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae

    Keogh, Michael-Christopher (et al.)

    Pages 1-16

  • Identifying PhosphoCTD-Associating Proteins

    Phatnani, Hemali P. (et al.)

    Pages 17-28

  • Imaging Alternative Splicing in Living Cells

    Wagner, Eric J. (et al.)

    Pages 29-46

  • Tobramycin Affinity Tag Purification of Spliceosomes

    Hartmuth, Klaus (et al.)

    Pages 47-64

  • Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing

    Celotto, Alicia M. (et al.)

    Pages 65-73

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-750-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-58829-225-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-61737-434-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
mRNA Processing and Metabolism
Book Subtitle
Methods and Protocols
Editors
  • Daniel R. Schoenberg
Series Title
Methods in Molecular Biology
Series Volume
257
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-750-5
DOI
10.1385/1592597505
Hardcover ISBN
978-1-58829-225-4
Softcover ISBN
978-1-61737-434-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 270
Topics