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  • Book
  • © 2003

Plant Functional Genomics

Methods and Protocols

Editors:

  1. Erich Grotewold

    1. Department of Plant Biology and Plant Biotechnology Center, The Ohio State University, Columbus

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 236)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
    PDF

  2. Finding Genes in Complex Plant Systems

    1. Front Matter

      Pages 1-1
      PDF

    2. An Improved Method for Plant BAC Library Construction

      • Meizhong Luo, Rod A. Wing
      Pages 3-19

    5. Precious Cells Contain Precious Information

      • Isabelle M. Henry, Dina F. Mandoli
      Pages 59-78

    6. Combined ESTs from Plant-Microbe Interactions

      • Edgar Huitema, Trudy A. Torto, Allison Styer, Sophien Kamoun
      Pages 79-83

  3. In Silico Prediction of Plant Gene Function

    1. Front Matter

      Pages 85-85
      PDF

  4. In Silico Prediction of Plant Gene Function

    1. Computer Software to Find Genes in Plant Genomic DNA

      • Ramana V. Davuluri, Michael Q. Zhang
      Pages 87-107

    2. Genomic Colinearity as a Tool for Plant Gene Isolation

      • Wusirika Ramakrishna, Jeffrey L. Bennetzen
      Pages 109-121

    3. Using Natural Allelic Diversity to Evaluate Gene Function

      • Sherry R. Whitt, Edward S. Buckler IV
      Pages 123-139

  5. Forward and Reverse Genetic Strategies

    1. Front Matter

      Pages 155-155
      PDF

    2. Transposon Tagging Using Activator (Ac) in Maize

      • Thomas P. Brutnell, Liza J. Conrad
      Pages 157-175

    3. T-DNA Mutagenesis in Arabidopsis

      • Jose M. Alonso, Anna N. Stepanova
      Pages 177-187

    4. Physical and Chemical Mutagenesis

      • Andrea Kodym, Rownak Afza
      Pages 189-203

    5. High-Throughput TILLING for Functional Genomics

      • Bradley J. Till, Trenton Colbert, Rachel Tompa, Linda C. Enns, Christine A. Codomo, Jessica E. Johnson et al.
      Pages 205-220

    6. Gene and Enhancer Traps for Gene Discovery

      • Marcela Rojas-Pierce, Patricia S. Springer
      Pages 221-240

    8. Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing

      • Susan Varsha Wesley, Qing Liu, Anna Wielopolska, Geoff Ellacott, Neil Smith, Surinder Singh et al.
      Pages 273-286
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About this book

Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.
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Reviews

"...provides in a single volume most of the commonly used approaches in plant genomics...worth reading and should interest a large public." - Proteomics

"This well written and illustrated guide reads easily and provides detailed protocols and an up-to-date bibliography for all reviewed strategies, making it the book that everybody in this field would need." - Cell Biochem Funct
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Editors and Affiliations

  • Department of Plant Biology and Plant Biotechnology Center, The Ohio State University, Columbus

    Erich Grotewold

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Bibliographic Information

  • Book Title: Plant Functional Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Erich Grotewold

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592594131

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-1-58829-145-5Published: 06 August 2003

  • Softcover ISBN: 978-1-61737-386-2Published: 01 December 2010

  • eBook ISBN: 978-1-59259-413-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 452

  • Topics: Plant Sciences

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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