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Methods in Molecular Biology

Protein'Protein Interactions

Methods and Applications

Editors: Fu, Haian (Ed.)

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eBook $169.00
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  • ISBN 978-1-59259-762-8
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Hardcover $219.99
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  • ISBN 978-1-58829-120-2
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Softcover $219.99
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  • ISBN 978-1-61737-373-2
  • Free shipping for individuals worldwide
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About this book

Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets.

Reviews

From the reviews:

"This book is thorough and packed with information...the volume is of high practical value and is fully recommended to all biochemists..." - Cell Biochemistry and Function

"The book is well-balanced and easy to use...well worth the purchasing cost." - ChemBioChem

"This … volume comprehensively describes a variety of biophysical-, cell biology- and molecular biology-based approaches to the analysis of protein-protein interactions. … This volume is recommended as a solid presentation on the fundamentals of protein interaction analysis, suitable for graduate biophysical chemistry courses and for those investigators new to the field who would like to get a solid grounding … ." (Wayne F. Patton, Proteomics, Issue 6, 2006)


Table of contents (35 chapters)

  • Structural Basis of Protein-Protein Interactions

    Liddington, Robert C.

    Pages 3-14

  • Quantitative Analysis of Protein-Protein Interactions

    Wilkinson, Keith D.

    Pages 15-31

  • Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry

    Velazquez-Campoy, Adrian (et al.)

    Pages 35-54

  • Circular Dichroism Analysis for Protein-Protein Interactions

    Greenfield, Norma J.

    Pages 55-77

  • Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy

    Gao, Guanghua (et al.)

    Pages 79-91

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-59259-762-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-58829-120-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • ISBN 978-1-61737-373-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein'Protein Interactions
Book Subtitle
Methods and Applications
Editors
  • Haian Fu
Series Title
Methods in Molecular Biology
Series Volume
261
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-59259-762-8
DOI
10.1385/1592597629
Hardcover ISBN
978-1-58829-120-2
Softcover ISBN
978-1-61737-373-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 532
Topics