Methods in Molecular Biology

Protein Purification Protocols

Editors: Cutler, Paul (Ed.)

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eBook $169.00
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  • ISBN 978-1-59259-655-3
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Hardcover $219.99
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Softcover $229.00
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  • ISBN 978-1-61737-334-3
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About this book

In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations.
Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.

Reviews

From reviews of the first edition...
"...very useful..."
-Angewandte Chemie: International Edition

"...valuable to keep around the laboratory..."
-Biopharm

"...recommended for protein chemists....There is something in this book for
everyone....an excellent working book as well as an excellent reference."
-JAOCS: Journal of the American Oil Chemists' Society

"...an excellent lab companion..."
-SIM News

"...a worthwhile purchase for novice and established practitioners alike."
-Microbiology Today

"...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists."
-Biomedical Chromatography

"Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterly

Table of contents (44 chapters)

  • General Strategies

    Doonan, Shawn (et al.)

    Pages 1-13

  • Preparation of Extracts From Animal Tissues

    Skehel, J. Mark

    Pages 15-20

  • Protein Extraction From Plant Tissues

    Fido, Roger J. (et al.)

    Pages 21-27

  • Extraction of Recombinant Protein From Bacteria

    McGettrick, Anne F. (et al.)

    Pages 29-35

  • Protein Extraction From Fungi

    Bridge, Paul D. (et al.)

    Pages 37-46

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-59259-655-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-58829-067-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $229.00
price for USA in USD
  • ISBN 978-1-61737-334-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Purification Protocols
Editors
  • Paul Cutler
Series Title
Methods in Molecular Biology
Series Volume
244
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-655-3
DOI
10.1385/159259655X
Hardcover ISBN
978-1-58829-067-0
Softcover ISBN
978-1-61737-334-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 484
Topics