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Protein Purification Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Paul Cutler

    1. Genomic and Proteomic Sciences Medicines Research Center, GlaxoSmithKline Pharmaceuticals, Stevenage, UK

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 244)

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Table of contents (44 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. General Strategies

    • Shawn Doonan, Paul Cutler
    Pages 1-13

  3. Preparation of Extracts From Animal Tissues

    • J. Mark Skehel
    Pages 15-20

  4. Protein Extraction From Plant Tissues

    • Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, Peter R. Shewry
    Pages 21-27

  5. Extraction of Recombinant Protein From Bacteria

    • Anne F. McGettrick, D. Margaret Worrall
    Pages 29-35

  6. Protein Extraction From Fungi

    • Paul D. Bridge, Tetsuo Kokubun, Monique S. J. Simmonds
    Pages 37-46

  7. Subcellular Fractionation of Animal Tissues

    • Norma M. Ryan
    Pages 47-52

  8. Subcellular Fractionation of Plant Tissues

    • Alyson K. Tobin, Caroline G. Bowsher
    Pages 53-63

  9. The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds

    • William S. Pierpoint
    Pages 65-74

  10. Avoidance of Proteolysis in Extracts

    • Robert J. Beynon, Simon Oliver
    Pages 75-84

  11. Concentration of Extracts

    • Shawn Doonan
    Pages 85-90

  12. Making and Changing Buffers

    • Shawn Doonan
    Pages 91-99

  13. Purification and Concentration by Ultrafiltration

    • Paul Schratter
    Pages 101-116

  14. Bulk Purification by Fractional precipitation

    • Shawn Doonan
    Pages 117-124

  15. Ion-Exchange Chromatography

    • Chris Selkirk
    Pages 125-131

  16. Hydrophobic Interaction Chromatography

    • Paul A. O’Farrell
    Pages 133-138

  17. Affinity Chromatography

    • Paul Cutler
    Pages 139-149

  18. Dye-Ligand Affinity Chromatography

    • Anne F. McGettrick, D. Margaret Worrall
    Pages 151-157

  19. Lectin Affinity Chromatography

    • Iris West, Owen Goldring
    Pages 159-166

  20. Immunoaffinity Chromatography

    • Paul Cutler
    Pages 167-177
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About this book

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Reviews

From reviews of the first edition...
"...very useful..."
-Angewandte Chemie: International Edition

"...valuable to keep around the laboratory..."
-Biopharm

"...recommended for protein chemists....There is something in this book for
everyone....an excellent working book as well as an excellent reference."
-JAOCS: Journal of the American Oil Chemists' Society

"...an excellent lab companion..."
-SIM News

"...a worthwhile purchase for novice and established practitioners alike."
-Microbiology Today

"...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists."
-Biomedical Chromatography

"Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterly

Editors and Affiliations

  • Genomic and Proteomic Sciences Medicines Research Center, GlaxoSmithKline Pharmaceuticals, Stevenage, UK

    Paul Cutler

Bibliographic Information

  • Book Title: Protein Purification Protocols

  • Editors: Paul Cutler

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259655X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-067-0Published: 12 December 2003

  • Softcover ISBN: 978-1-61737-334-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-655-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 484

  • Topics: Biochemistry, general

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