Methods in Molecular Biology

Capillary Electrophoresis of Proteins and Peptides

Editors: Strege, Mark A., Lagu, Avinash L. (Eds.)

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About this book

Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis

    Pages 1-14

    Lucy, Charles A. (et al.)

  • Capillary Coating for Protein Separation Based on Si-O and Si-C Covalent Bond Formation for Capillary Electrophoresis With Laser-Induced Fluorescence Detection

    Pages 15-27

    Ahmadzadeh, Hossein (et al.)

  • On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection

    Pages 29-38

    Ahmadzadeh, Hossein (et al.)

  • Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications

    Pages 39-75

    Sowell, John (et al.)

  • Capillary Electrophoresis in the Analysis and Monitoring of Biotechnological Processes

    Pages 77-120

    Klyushnichenko, Vadim

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59259-798-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-017-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • ISBN 978-1-61737-307-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Capillary Electrophoresis of Proteins and Peptides
Editors
  • Mark A. Strege
  • Avinash L. Lagu
Series Title
Methods in Molecular Biology
Series Volume
276
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-59259-798-7
DOI
10.1385/159259798X
Hardcover ISBN
978-1-58829-017-5
Softcover ISBN
978-1-61737-307-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 332
Topics