Methods in Molecular Biology

Platelets and Megakaryocytes

Volume 2: Perspectives and Techniques

Editors: Gibbins, Jonathan M., Mahaut-Smith, Martyn P. (Eds.)

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  • ISBN 978-1-59259-783-3
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About this book

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function.
Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Reviews

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology

Table of contents (29 chapters)

Table of contents (29 chapters)

Buy this book

eBook $189.00
price for USA in USD
  • ISBN 978-1-59259-783-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
  • Institutional customers should get in touch with their account manager
Hardcover $279.99
price for USA in USD
Softcover $249.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Platelets and Megakaryocytes
Book Subtitle
Volume 2: Perspectives and Techniques
Editors
  • Jonathan M. Gibbins
  • Martyn P. Mahaut-Smith
Series Title
Methods in Molecular Biology
Series Volume
273
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-783-3
DOI
10.1385/1592597831
Hardcover ISBN
978-1-58829-011-3
Softcover ISBN
978-1-61737-305-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XX, 504
Number of Illustrations
108 b/w illustrations, 5 illustrations in colour
Topics