Methods in Molecular Biology

Molecular Cytogenetics

Protocols and Applications

Editors: Fan, Yao-Shan (Ed.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59259-300-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-006-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • ISBN 978-1-61737-300-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations.
Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.

Reviews

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent.

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics

Table of contents (27 chapters)

  • Molecular Cytogenetics in Medicine

    Fan, Yao-Shan

    Pages 3-20

  • Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets

    Morrison, Larry E. (et al.)

    Pages 21-40

  • Labeling Fluorescence In Situ Hybridization Probes for RNA Targets

    Ramakrishnan, Ramesh (et al.)

    Pages 41-49

  • Basic FISH Techniques and Troubleshooting

    Stedum, Sue (et al.)

    Pages 51-63

  • Chromosome Microdissection

    Guan, Xin-Yuan

    Pages 67-76

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59259-300-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-58829-006-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • ISBN 978-1-61737-300-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Molecular Cytogenetics
Book Subtitle
Protocols and Applications
Editors
  • Yao-Shan Fan
Series Title
Methods in Molecular Biology
Series Volume
204
Copyright
2003
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-300-2
DOI
10.1385/1592593003
Hardcover ISBN
978-1-58829-006-9
Softcover ISBN
978-1-61737-300-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 414
Topics