Plant and Food Carotenoids
Methods and Protocols
Editors: Concepción, Manuel Rodríguez, Welsch, Ralf (Eds.)
Free Preview- Includes cutting- edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant and Food Carotenoids: Methods and Protocols aims to be helpful to researchers of other disciplines that are impacted by carotenoids, including photosynthesis, biotechnology, food science, and nutrition.
- Table of contents (30 chapters)
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Pathways for Carotenoid Biosynthesis, Degradation, and Storage
Pages 3-23
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A Simple In Vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-Chain Prenyltransferases
Pages 27-38
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Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro
Pages 39-52
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Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO
Pages 53-61
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Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases
Pages 63-74
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Table of contents (30 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Plant and Food Carotenoids
- Book Subtitle
- Methods and Protocols
- Editors
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- Manuel Rodríguez Concepción
- Ralf Welsch
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2083
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9952-1
- DOI
- 10.1007/978-1-4939-9952-1
- Hardcover ISBN
- 978-1-4939-9951-4
- Softcover ISBN
- 978-1-4939-9954-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 421
- Number of Illustrations
- 23 b/w illustrations, 58 illustrations in colour
- Topics
