Methods in Molecular Biology

Macrophage Migration Inhibitory Factor

Methods and Protocols

Editors: Harris, James, Morand, Eric F. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9936-1
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Hardcover $169.99
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About this book

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. 


Table of contents (22 chapters)

Table of contents (22 chapters)
  • Studying the Pro-Migratory Effects of MIF

    Pages 1-18

    Hoffmann, Adrian (et al.)

  • In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions Under Flow Conditions

    Pages 19-25

    Hickey, Michael J.

  • Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo

    Pages 27-37

    Norman, M. Ursula (et al.)

  • Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells

    Pages 39-46

    Lang, Tali

  • Measuring MIF in Biological Fluids

    Pages 47-56

    Vincent, Fabien B. (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9936-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-9935-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Macrophage Migration Inhibitory Factor
Book Subtitle
Methods and Protocols
Editors
  • James Harris
  • Eric F. Morand
Series Title
Methods in Molecular Biology
Series Volume
2080
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9936-1
DOI
10.1007/978-1-4939-9936-1
Hardcover ISBN
978-1-4939-9935-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 265
Number of Illustrations
9 b/w illustrations, 34 illustrations in colour
Topics