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Macrophage Migration Inhibitory Factor

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. James Harris

    1. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, Australia

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  2. Eric F. Morand

    1. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2080)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
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  2. Studying the Pro-Migratory Effects of MIF

    • Adrian Hoffmann, Leon Christian Zwißler, Omar El Bounkari, Jürgen Bernhagen
    Pages 1-18

  3. In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions Under Flow Conditions

    • Michael J. Hickey
    Pages 19-25

  4. Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo

    • M. Ursula Norman, Michael J. Hickey
    Pages 27-37

  5. Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells

    • Tali Lang
    Pages 39-46

  6. Measuring MIF in Biological Fluids

    • Fabien B. Vincent, Tali Lang
    Pages 47-56

  7. Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif−/− Mice

    • Jacqueline K. Flynn, Nadia S. Deen, James Harris
    Pages 57-66

  8. Genotyping Two Promoter Polymorphisms in the MIF Gene: A −794 CATT5–8 Microsatellite Repeat and a −173 G/C SNP

    • Lin Leng, Edwin Siu, Richard Bucala
    Pages 67-84

  9. Staining MIF in Cells for Confocal Microscopy

    • James Harris
    Pages 85-91

  10. Microscopy Methods for Imaging MIF and Its Interaction Partners

    • Kirstin D. Elgass, Sarah J. Creed, Ina Rudloff
    Pages 93-114

  11. Co-Immunoprecipitation of Macrophage Migration Inhibitory Factor

    • Jawad H. Abidi, James Harris, Nadia S. Deen
    Pages 115-122

  12. Concurrent Immunohistochemical Localization and Western Blot Analysis of the MIF Receptor, CD74, in Formalin-Fixed, Paraffin-Embedded Tissue

    • Amanda Graham, Warren B. Nothnick
    Pages 123-134

  13. Methods to Determine the Effects of MIF on In Vitro Osteoclastogenesis Using Murine Bone Marrow-Derived Cells and Human Peripheral Blood Mononuclear Cells

    • Ran Gu
    Pages 135-145

  14. Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF

    • Nadia S. Deen, Jacinta P. Lee, James Harris
    Pages 147-158

  15. Assays for Measuring the Role of MIF in NLRP3 Inflammasome Activation

    • Anita A. Pinar, James Harris
    Pages 159-172

  16. Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release

    • Shahrzad Zamani, Eric F. Morand, Jacqueline K. Flynn
    Pages 173-183

  17. Assessing the Role of MIF in Plasmodium spp. Infections Using Ex Vivo Models

    • Elizabeth H. Aitken
    Pages 185-192

  18. The Effect of Macrophage Migration Inhibitory Factor on Intestinal Permeability: FITC-Dextran Serum Measurement and Transmission Electron Microscopy

    • Milica Vujičić, Sanja Despotović, Tamara Saksida, Ivana Stojanović
    Pages 193-201

  19. Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma

    • Venkata Sita Rama Raju Allam, Maria B. Sukkar
    Pages 203-212

  20. Investigating MIF in Mouse Model of Gout

    • Izabela Galvão, Allysson Cramer, Flavio Almeida Amaral
    Pages 213-222
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Keywords

About this book

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. 



Editors and Affiliations

  • Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, Australia

    James Harris, Eric F. Morand

Bibliographic Information

  • Book Title: Macrophage Migration Inhibitory Factor

  • Book Subtitle: Methods and Protocols

  • Editors: James Harris, Eric F. Morand

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9936-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9935-4Published: 19 November 2019

  • Softcover ISBN: 978-1-4939-9938-5Published: 19 November 2020

  • eBook ISBN: 978-1-4939-9936-1Published: 19 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 265

  • Number of Illustrations: 9 b/w illustrations, 34 illustrations in colour

  • Topics: Immunology, Cell Biology, Molecular Medicine

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