Methods in Molecular Biology

Chimeric RNA

Methods and Protocols

Editors: Li, Hui, Elfman, Justin (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-4939-9904-0
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Hardcover $199.99
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About this book

This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Prediction, Characterization, and In Silico Validation of Chimeric RNAs

    Pages 3-12

    Singh, Sandeep (et al.)

  • Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope

    Pages 13-25

    Vellichirammal, Neetha Nanoth (et al.)

  • Identification of Chimeric RNAs Using RNA-Seq Reads and Protein–Protein Interactions of Translated Chimeras

    Pages 27-40

    Frenkel-Morgenstern, Milana

  • Gene Fusion Discovery with INTEGRATE

    Pages 41-68

    Zhang, Jin (et al.)

  • Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit

    Pages 69-79

    Vats, Pankaj (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9904-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9903-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chimeric RNA
Book Subtitle
Methods and Protocols
Editors
  • Hui Li
  • Justin Elfman
Series Title
Methods in Molecular Biology
Series Volume
2079
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9904-0
DOI
10.1007/978-1-4939-9904-0
Hardcover ISBN
978-1-4939-9903-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 262
Number of Illustrations
4 b/w illustrations, 52 illustrations in colour
Topics