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Methods in Molecular Biology

Histidine Phosphorylation

Methods and Protocols

Editors: Eyers, Claire E. (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts 
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9884-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-9883-8
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field. 

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Table of contents (16 chapters)

Table of contents (16 chapters)
  • Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems

    Pages 1-18

    Trajtenberg, Felipe (et al.)

  • A High-Throughput Strategy for Recombinant Protein Expression and Solubility Screen in Escherichia coli : A Case of Sensor Histidine Kinase

    Pages 19-36

    Szmitkowska, Agnieszka (et al.)

  • SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation

    Pages 37-49

    Fischer, Jonathan T. (et al.)

  • A Quantitative Method for the Measurement of Protein Histidine Phosphorylation

    Pages 51-61

    Attwood, Paul V.

  • Analysis of 1- and 3-Phosphohistidine (pHis) Protein Modification Using Model Enzymes Expressed in Bacteria

    Pages 63-81

    Clubbs Coldron, Alice K. M. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9884-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-9883-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Histidine Phosphorylation
Book Subtitle
Methods and Protocols
Editors
  • Claire E. Eyers
Series Title
Methods in Molecular Biology
Series Volume
2077
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9884-5
DOI
10.1007/978-1-4939-9884-5
Hardcover ISBN
978-1-4939-9883-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 256
Number of Illustrations
10 b/w illustrations, 44 illustrations in colour
Topics