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Type 2 Diabetes

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Claire J. Stocker

    1. Medical School, University of Buckingham, Buckingham, UK

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  • Includes cuttting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2076)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now?

    • John C. Clapham
    Pages 1-30

  3. Practical Considerations for In Vivo Mouse Studies

    • Edward T. Wargent
    Pages 31-42

  4. Nutritional Models of Type 2 Diabetes Mellitus

    • Beverly Sara Mühlhäusler, Carla Toop, Sheridan Gentili
    Pages 43-69

  5. Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes

    • Paul W. Finn
    Pages 71-84

  6. Whole-Exome Sequencing (WES) for Illumina Short Read Sequencers Using Solution-Based Capture

    • Milind C. Mahajan, Andrew S. McLellan
    Pages 85-108

  7. Gene Expression Mining in Type 2 Diabetes Research

    • Donald R. Dunbar
    Pages 109-117

  8. Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes

    • Maysson Ibrahim
    Pages 119-128

  9. Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia

    • Jayne A. L. Houghton
    Pages 129-177

  10. Isolation and Purification of Rodent Pancreatic Islets of Langerhans

    • Jacqueline F. O’Dowd, Claire J. Stocker
    Pages 179-184

  11. Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry

    • Matthew J. Butcher, Michelle B. Trevino, Yumi Imai, Elena V. Galkina
    Pages 185-197

  12. Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation

    • Ionel Sandovici, Lisa M. Nicholas, Laura P. O’Neill
    Pages 199-213

  13. Quantification of Pancreatic Islets: Using Image Analysis Tools

    • Parvathy E. Harikumar
    Pages 215-229

  14. Measurement of Calcium Signaling in Beta-Cell Lines Using Epifluorescence and Confocal Microscopy

    • Joanne L. Selway
    Pages 231-239

  15. Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells

    • Rodrigo Carlessi, Vinicius Cruzat, Younan Chen, Philip Newsholme
    Pages 241-253

  16. Primary Adipocytes as a Model for Insulin Sensitivity

    • Mohamed S. Zaibi
    Pages 255-263

  17. Assessing Islet Transplantation Outcome in Mice

    • Aileen J. F. King, Chloe L. Rackham
    Pages 265-280

  18. Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent

    • Edward T. Wargent
    Pages 281-297

  19. Back Matter

    Pages 299-300
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Keywords

About this book

This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field. 

Editors and Affiliations

  • Medical School, University of Buckingham, Buckingham, UK

    Claire J. Stocker

Bibliographic Information

  • Book Title: Type 2 Diabetes

  • Book Subtitle: Methods and Protocols

  • Editors: Claire J. Stocker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9882-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9880-7Published: 05 October 2019

  • eBook ISBN: 978-1-4939-9882-1Published: 04 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 300

  • Number of Illustrations: 18 b/w illustrations, 32 illustrations in colour

  • Topics: Molecular Medicine

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