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Horizontal Gene Transfer

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Fernando de la Cruz

    1. Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-CSIC, Santander, Spain

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2075)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
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  2. Methods for the Detection and Isolation of Mobile Genetic Elements

    1. Front Matter

      Pages 1-1
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    2. Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples

      • Kaitlin A. Tagg, Carola Venturini, Muhammad Kamruzzaman, Andrew N. Ginn, Sally R. Partridge
      Pages 3-20

    3. Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health

      • Ana R. Freitas, Carla Novais, Luísa Peixe, Teresa M. Coque
      Pages 21-38

    4. Detection, Isolation, and Characterization of Plasmids in the Environment

      • Khald Blau, Sven Jechalke, Kornelia Smalla
      Pages 39-60

    5. Bacteriophage Isolation and Characterization: Phages of Escherichia coli

      • Juan Jofre, Maite Muniesa
      Pages 61-79

    6. Detection and Characterization of Transposons in Bacteria

      • Catherine Guynet, Phan Thai Nguyen Le, Michael Chandler, Bao Ton-Hoang
      Pages 81-90

  3. Methods to Quantify and Characterize Horizontal Gene Transfer

    1. Front Matter

      Pages 91-91
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    2. Measuring Plasmid Conjugation Using Antibiotic Selection

      • Jorge Rodriguez-Grande, Raul Fernandez-Lopez
      Pages 93-98

    3. Measuring Plasmid Conjugation Using Fluorescent Reporters

      • Carolina Palencia-Gandara, Raul Ruiz, Raul Fernandez-Lopez
      Pages 99-110

    4. Methods to Quantify DNA Transfer in Enterococcus

      • Carla Novais, Ana R. Freitas, Ricardo León-Sampedro, Luísa Peixe, Teresa M. Coque
      Pages 111-122

    5. Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis

      • Ryan R. Clark, Todd A. Gray, Keith M. Derbyshire
      Pages 123-134

    6. Spectrophotometric Assays to Quantify the Activity of T4SS ATPases

      • Elena Cabezón, Ignacio Arechaga
      Pages 135-143

    7. Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis

      • María Lucas, Gabriel Moncalián
      Pages 145-156

    8. First Biochemical Steps on Bacterial Transposition Pathways

      • Catherine Guynet, Emilien Nicolas, Bao Ton-Hoang, Jean-Yves Bouet, Bernard Hallet
      Pages 157-177

    9. Natural Transformation in Escherichia coli

      • Yoko Komiyama, Sumio Maeda
      Pages 179-187

    10. Integron Identification in Bacterial Genomes and Cassette Recombination Assays

      • Claire Vit, Céline Loot, José Antonio Escudero, Aleksandra Nivina, Didier Mazel
      Pages 189-208

    11. Methods to Identify and Analyze Vesicle-Protected DNA Transfer

      • Alba Blesa, José Berenguer
      Pages 209-221

    12. Measuring Plasmid Stability in Gram-Negative Bacteria

      • Damián Lobato-Márquez
      Pages 223-233

    13. Methods for the Analysis and Characterization of Defense Mechanisms Against Horizontal Gene Transfer: CRISPR Systems

      • Alicia Calvo-Villamañán, Aude Bernheim, David Bikard
      Pages 235-249
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Keywords

About this book

This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality.

Editors and Affiliations

  • Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-CSIC, Santander, Spain

    Fernando de la Cruz

Bibliographic Information

  • Book Title: Horizontal Gene Transfer

  • Book Subtitle: Methods and Protocols

  • Editors: Fernando de la Cruz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9877-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9876-0Published: 04 October 2019

  • Softcover ISBN: 978-1-4939-9879-1Published: 04 October 2020

  • eBook ISBN: 978-1-4939-9877-7Published: 04 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 413

  • Number of Illustrations: 10 b/w illustrations, 52 illustrations in colour

  • Topics: Microbial Genetics and Genomics, Bacteriology

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