Horizontal Gene Transfer
Methods and Protocols
Editors: de la Cruz, Fernando (Ed.)
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- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality.
- Table of contents (28 chapters)
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Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples
Pages 3-20
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Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health
Pages 21-38
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Detection, Isolation, and Characterization of Plasmids in the Environment
Pages 39-60
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Bacteriophage Isolation and Characterization: Phages of Escherichia coli
Pages 61-79
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Detection and Characterization of Transposons in Bacteria
Pages 81-90
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Table of contents (28 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Horizontal Gene Transfer
- Book Subtitle
- Methods and Protocols
- Editors
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- Fernando de la Cruz
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2075
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9877-7
- DOI
- 10.1007/978-1-4939-9877-7
- Hardcover ISBN
- 978-1-4939-9876-0
- Softcover ISBN
- 978-1-4939-9879-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 413
- Number of Illustrations
- 10 b/w illustrations, 52 illustrations in colour
- Topics
