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Methods in Molecular Biology

Toxoplasma gondii

Methods and Protocols

Editors: Tonkin, Christopher J. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9857-9
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About this book

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.


Table of contents (23 chapters)

Table of contents (23 chapters)
  • What a Difference 30 Years Makes! A Perspective on Changes in Research Methodologies Used to Study Toxoplasma gondii

    Pages 1-25

    Boothroyd, John C.

  • ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms

    Pages 27-47

    Harb, Omar S. (et al.)

  • Isolation and Genotyping of Toxoplasma gondii Strains

    Pages 49-80

    Su, Chunlei (et al.)

  • Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation

    Pages 81-98

    Sokol, Sarah L. (et al.)

  • Assays for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse

    Pages 99-116

    Wang, Qiuling (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-9857-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9856-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Toxoplasma gondii
Book Subtitle
Methods and Protocols
Editors
  • Christopher J. Tonkin
Series Title
Methods in Molecular Biology
Series Volume
2071
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9857-9
DOI
10.1007/978-1-4939-9857-9
Hardcover ISBN
978-1-4939-9856-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 470
Number of Illustrations
14 b/w illustrations, 63 illustrations in colour
Topics

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